Differential analysis of three data matrices across conditions. Volcano plots corresponding to four comparisons by ANOVA test in (a) total cellular lysate (b) mitochondria-enriched fractions and (c) mtFE data. Differentially expressed proteins (adj. p-value < 0.05) can be seen in dark red (lysate), dark green (mito) or dark violet (mtFE scores). Pastel-colored points correspond to moderate protein changes (adjusted p between 0.05 and 0.1), while light gray points represent unaffected liver proteins. The comparisons between conditions correspond either to differences in genotype or cancer state. The N represents the number of significantly changed proteins (adj. p-value < 0.05) for each comparison. (d) Venn diagram results and its summary graph of shared significantly regulated proteins between three datasets (ANOVA test, adj. p-value < 0.05). (e) Mapping of KEGG cellular pathway database with significant proteins from the three datasets. Y-axis represents the percentage of total mapped proteins in KEGG database that corresponds to specific cellular pathway on X-axis.