Quantifying the impacts of climate change on prehistoric demography is crucial for understanding the adaptive pathways taken by human populations. Archaeologists across South America have pointed to patterns of regional abandonment during the Middle Holocene (8200 to 4200 cal BP) as evidence of sensitivity to shifts in hydroclimate over this period. We develop a unified approach to investigate demography and climate in South America and aim to clarify the extent to which evidence of local anthropic responses can be generalised to large-scale trends. We achieve this by integrating archaeological radiocarbon data and palaeoclimatic time series to show that population decline occurred coeval with the transition to the initial mid-Holocene across South America. Through the analysis of radiocarbon dates with Monte Carlo methods, we find multiple, sustained phases of downturn associated to periods of high climatic variability. A likely driver of the duration and severity of demographic turnover is the frequency of exceptional climatic events, rather than the absolute magnitude of change. Unpredictable levels of tropical precipitation had sustained negative impacts on pre-Columbian populations lasting until at least 6000 cal BP, after which recovery is evident. Our results support the inference that a demographic regime shift in the second half of the Middle Holocene were coeval with cultural practices surrounding Neotropical plant management and early cultivation, possibly acting as buffers when the wild resource base was in flux.
The initial human colonisation of South America was a rapid process that led to the dispersal of hunter-gatherer populations to every major biome on the continent within a few millennia, starting at the latest around 14 k calendar years before present (cal BP). Colonising groups successfully adapted to a broad range of environments during the Terminal Pleistocene and early Holocene, from the Amazonian rainforest and Patagonian grasslands to the high Andes1,2,3,4. The genetic and demographic structure of early populations have been the focus of substantial recent research5,6. In parallel, a growing body of archaeological evidence from several regions has suggested that climatic transitions acted as a driver of significant regional depopulation during the mid-Holocene. Discontinuities in archaeological records have specifically been linked to increasingly unpredictable climatic regimes around this transition. Abandonment or retreat to refugia is suggested to have occurred in central Amazonia7, the south-central Cordillera8,9, eastern Brazil10, the Sabana de Bogotá11 and the Puna de Atacama12. The inferred existence of mid-Holocene demographic regime shifts in these widely distributed environments indicates that exogenous factors influenced human populations across the continent concurrently at this time.
Here we investigate pre-Columbian demographic dynamics to investigate the resilience of early South American foraging adaptations to periods of abrupt climate change. We focus specifically on the initial transition to the Middle Holocene (8.2–4.2 k cal BP13), during which South America was characterised by overall more arid conditions14. Large-scale analyses using of South American radiocarbon data as a population proxy have previously noted exceptionally low relative population around ~8.2 k cal BP5. We posit a connection between sudden, high-amplitude alterations to hydroclimate and widespread archaeological evidence of upheaval among human populations associated to the mid-Holocene transition. Globally, demographic overturn together with climate change has been suggested as a major driver of prehistoric culture change over this interval, with radiocarbon data proving especially instrumental in this regard15,16,17,18. In South America, the broad range of research intensities, historical trends in scholarship, preservation conditions, and site formation processes, against the backdrop of its cultural and ecological diversity3, requires any analysis to be undertaken in explicitly quantitative terms. We attend to this by assessing relative change in prehistoric South American demography using summed probability distributions of calibrated radiocarbon dates (Fig. 1, hereafter SPDs) combined with Monte Carlo simulation as an indirect proxy for demographic patterns over time19,20,21. To contextualise these findings, we also identify the frequency of hydroclimatic anomalies during the mid-Holocene across multiple palaeoclimatic time series (Methods), with the assumption that ecological shifts of direct consequence to human adaptations will track hydroclimatic regimes.
We initially test the radiocarbon record of South America against the same null hypothesis of exponential growth for the period 12–2 k 14C years before present, based on an assessment of curve shape and goodness-of-fit. Following the detection of statistically significant negative departures from this model starting at ~8.2 k cal BP, we expand our approach through a two-phase demographic model with the goal of pinpointing where a demographic regime change is most likely to start (Fig. 2). Next, following preceding research22, we define our mid-Holocene demographic expectations with reference to prevailing dynamics prior to the phase of decline in order to condition our expectations of what constitutes a significant departure against the prevailing trend identified in the prior regime.
We also disaggregate the data into three regions for further analyses, comprised of: (a) the northern and central Andean Highlands, foothills, and foreland basin, (b) the tropical Lowlands of Amazonia and circum-Amazonia, and (c) the Southern Cone, incorporating the southern Andes, Pampas, and Patagonia. These subdivisions target variation in the structure of the archaeological data within broad topographic, biogeographical, and climatic realms. With reference to the systems driving rainfall variability across South America (Supplementary Information), these regions respectively approximate areas principally influenced by moisture transportation from the Atlantic, areas influenced by the Atlantic and potentially Pacific sources, and predominantly South Pacific/South Atlantic-influenced zones13,23,24,25. The Southern Cone, as defined here, also acts as a geographical proxy for the southern limits of tropical domesticates prior to the beginning of the late Holocene6,26. We take consistent patterns in the radiocarbon data to reflect robust and independent trends in human population across regions rather than specific archaeological cultures. These patterns permit identification of demographic sensitivity to climatic variability on a scale below that of the continent as a whole (Methods). We discuss the behavioural and social consequences of climatic variability during the mid-Holocene for the pre-Columbian population of South America based on the available archaeological data, as well as the role of climate as a driver of cultural change in general.
Our results show that the demographic trend for South America falls significantly below expectations for population growth after 8.6 k cal BP. After this point in time, periodic and statistically significant population deflation in the archaeological 14C record is apparent on a continental level, lasting at least until 6 k cal BP (p < 0.001, Fig. 2, left). This indicates that exceptional deviations from early Holocene demographic regimes occurred. The millennial-scale downturns associated to the initial Middle Holocene can be subdivided into three phases beginning with the initial dip in the demographic proxy at ~8.6 k cal BP, followed by ~7.7 k cal BP and finally ~6.9 k cal BP. These are bracketed by brief periods of recovery lasting two centuries or less. After the initial mid-Holocene, there is a return to model expectations, which are exceeded by ~5.3 k cal BP, likely marking the transition to a new demographic regime after ~6 k cal BP5. Our index of variability (Fig. 2, right) is derived from a robust outlier analysis of multiple palaeoclimatic records that together provide spatial coverage of precipitation patterns across South America. Our summary index shows a rapid increase starting at 8.6 k cal BP, concurrent with the first phase of downturn, peaking more than two standard deviations above the dataset mean at ~8.4 and 8.2–8.1 k cal BP. Following a short hiatus coincident with recovery, a second spike prefaces a second bout of demographic reduction after ~7.7 k cal BP. A third (~6.9–6 k cal BP) phase of sustained population decline spans the transition to more arid conditions that are typically recognised in the mid-Holocene of South America23. This is visible as a trough in our variability index punctuated by less intense yet above-average variability around 7.2 k and 6.5 k cal BP. Placing the frequency of hydroclimatic anomalies before and during the mid-Holocene transition alongside our demographic proxy illustrates the extent to which these patterns are coeval in time.
The next set of analyses aims to identify spatial variability in demographic dynamics associated to the middle Holocene. Disaggregating the archaeological 14C record into three regions formally describes significant variation in the distribution and intensity of demographic downturns, underscoring that the impacts of climatic variability are themselves variable in space (Fig. 3). Negative and positive deviations in the permutation tests reflect periods where subsets of the data significantly exceed the overall continental trend27, indirectly confirming the existence of a continent-wide decline starting at ~8.6 k cal BP. It is important to note the reason for conformity between the summed probability distributions around 8.2 k cal BP. We find a lack of significant downturn in relation to the overall trend in the continental dataset consistent with the South America-wide downturn identified separately (see Fig. 2). As indicated by the null model test, the entire modelling domain is experiencing a demographic contraction at this time, which masks the statistical distinctiveness of coeval downturns in subsets of the data. The absence of a significant negative signal at this time in this test is therefore to be expected.
Together, the permutation tests reveal a staggered temporal structure in the summed probability distributions over the mid-Holocene chron. The tropical Highlands and Lowlands trends both show significant yet out of phase negative deviations from the continental confidence envelope. The Highlands appear responsive to heightened aridity around ~6 k and ~5 k cal BP, while declines in the Lowlands appear around 7 k cal BP. Inverse demographic trends exist between the Lowlands and Southern Cone at this time and between the Highlands and Southern Cone around 6 k cal BP. We observe that the most sustained local deviations in the Highland and Southern Cone data occur after 6 k cal BP, albeit opposite trends (negative and positive, respectively), while the Lowlands experiences only centennial-scale negative deviations. Pairwise regional comparisons with permutation tests (Fig. S2) concordantly show that the tropical Lowland and Highland SPDs return non-significant p-values, indicating statistical similarity, while both are significantly different from the Southern Cone. Together, our results indicate that a common mechanism may have influenced tropical South America, separately from the subtropical and temperate biomes of the Southern Cone. To this effect, we note significantly higher relative population in this region already by 7.5 k cal BP and repeatedly thereafter, with none of the negative phases experienced elsewhere.
In summary, we performed a parameter sweep on a broad range of possible null models for the Early and Middle Holocene. This indicated a critical breakpoint occurred at 8.6 k cal BP. To investigate the degree of departure from this stable (weakly linear) post-colonisation trend, we conditioned our null model on this data. When tested against simulated radiocarbon data generated with Monte Carlo methods, our results show a repeated and statistically significant downturn of varying duration and intensity after 8.6 k cal BP, concurrent with increasing climatic variability from this point in time. These phases last until at least 6 k cal BP, at which point a second regime shift appears likely5. Further testing reveals centennial-scale depressions in the archaeological 14C record are present across highly diverse environmental and cultural settings, revealing widespread population decline during the mid-Holocene. Regional differences are detected through comparison with the structure of the continent-wide dataset (Fig. 3), and identifies separate phases of downturn in the tropical Highlands and Lowlands data. In contrast, the Southern Cone remains largely above or within the continental confidence envelope. In statistical terms, the tropical Highlands and Lowlands reveal approximately equivalent demographic trends with some local differences, while the Southern Cone is significantly different from both. Significant positive divergences from the continental trend suggests that this region apparently did not suffer the same degree of downturn during periods of high climatic variability. Accounting for time-dependent site loss and spatial variability in preservation and research intensity (Methods), the weight of the statistical evidence suggests that the depth of the mid-Holocene downturn reflects much more than an oscillation of local population levels around a stable mean5. Rather, our results indicate that a phased demographic contraction over a period of several centuries took place across South America. Strong synchrony is evident between all three regions following peak climatic variability (Fig. 2). After the initial mid-Holocene, however, regional demographic responses diverge. Below we discuss possible impacts and consequences of this pattern in terms of the social and bioclimatic changes experienced by indigenous South Americans during this period of interest.
An initial period of high climatic variability spans the transition from the early to the middle Holocene. Three steps (8.4k, 8.2k, and 8.1 k cal BP) have an exceptionally high frequency of anomalies and the ramping up of frequent anomalous events shown in our index (Fig. 2) correlates with the initial drop in relative population observed across South America at and after 8.6 k cal BP. Archaeologists have repeatedly pointed to mid-Holocene aridity across South America as a mechanism driving occupational hiatuses in multiple localities, indicative of abandonment or logistic range reductions tethered to more predictable resources7,8,9,10,11,12,27. As illustrated by our archaeological summed probability distributions, significant departures from the quasi-stable Early Holocene regime occurred at least until 6 k cal BP, in the form of several protracted periods of population decline. A second, attenuated phase of anomalous climatic events following the initial extreme phase reveals the continuing impact of climatic variability on human populations. Our results indicate that precipitation variability, as well as absolute reductions in moisture, may have acted as joint drivers of demographic change in leading up to and in the first half of the Middle Holocene28,29. Importantly, the point identified as a probable demographic regime shift here is independent of the palaeoclimatic records included in our index.
A key influence on summer monsoon precipitation is the seasonal procession of the Intertropical Convergence Zone (ITCZ) and its interaction with the South Atlantic Convergence Zone (SACZ) over tropical South America. As the magnitude of ITCZ movement southwards is ultimately driven by orbitally-forced changes in North Atlantic surface temperature, negative anomalies result in a northerly (<10 °S) mean latitude of the ITCZ23. This leads to a reduction in precipitation in eastern Brazil and southern Amazonia, generally in antiphase to wetter conditions in the northern and western portions of the continent during such events, including the tropical Andes24,30. Our selection of proxies (Fig. S3) provides a long-term average of variability in this mechanism in latitudinal cross-section across tropical South America, as well as a separate index of precipitation in the southern mid-latitudes of the continent, which are predominantly influenced by the relative strength of Pacific westerlies31. Furthermore, simulated precipitation grids (Fig. 4, top) suggest that the highest variance at the start of the mid-Holocene occurred in a broad arc across the tropics, from the north of the continent to eastern Brazil, inflected via western Amazonia. The central Amazon and circumscribed areas of the Pacific coast experienced the least variability in tropical precipitation patterns over this period. The latter agrees with the suppression of the ENSO phenomenon in the Pacific during the mid-Holocene32, although overall the southern latitudes of the continent present the least variable precipitation patterns. Although flora responded to overall more xeric mid-Holocene conditions, biome-scale vegetational transitions appear not to have been severe when averaged over approximately four millennia30,33,34. Foragers adapted to the diverse terminal- and post-glacial environments of South America2,4 consequently also reacted in varying ways.
We propose that a combination of factors was responsible for sustained demographic downturn evident in the summed probability distributions. Relatively sudden, high-amplitude variability in precipitation patterns acted as “climatic shocks”35 that provoked the downturns observable in our demographic proxy (Figs 2–4). Following initial spikes in climatic variability, depressed tropical moisture availability in the mid-Holocene23,36 may have caused small changes in precipitation to have disproportionate impacts on human-occupied niches. Populations that were adapted to early Holocene conditions may have first suffered negative impacts to their resilience, translating to increased vulnerability to comparatively small-scale climatic events after 8.2 k cal BP. Concordantly, where climatic instability is less pervasive, for example in the Southern Cone as defined here (Fig. 4, bottom), the negative effects on demography are lessened in comparison to regions where some degree of climatic oscillations endured, as in the tropical Highlands and Lowlands. Nonetheless, after 6 k cal BP, our archaeological 14C proxy in tropical regions is sufficiently recovered to be consistent with, or exceed, model expectations. The continental confidence envelope is surpassed in the Highlands by the onset of the early Holocene at 4.2 k cal BP, while the Lowlands do so well before this point in time (Fig. 3).
Amidst the geographically-variable effects of climate change, South American populations themselves enacted modifications to local ecosystems through the deployment of plant management and cultivation practices37,38,39,40. The precocious use and dispersal of comestible and useful plants began in the Early Holocene in South America, and the long-distance translocation of crops such as peanut (Arachis hypogaea), manioc (Manihot esculenta), and maize (Zea mays) by the mid-Holocene40,41 highlights the deep antiquity of cultivation practices in the Neotropics. These and other species were integrated into diversified and likely regionally-specific multi-crop procurement systems at various times after 8.2 k cal BP37,39,42. The deliberate and incidental use of fire to modify local ecologies also influenced and shaped the environmental niches of which these crops were a part43,44,45,46. We suggest that the development of these intertropical systems is a crucial development in the context of significant population reduction relative to earlier periods. Under the stresses induced by both demographic and environmental conditions, anthropic ecologies that already incorporated cultivated or managed resources before the mid-Holocene may have become proportionately more important to subsistence strategies in the face of an unstable climate28,47. The increasing visibility of cultivated plants in the palaeobotanical record from the mid-Holocene could suggest that climate change may have promoted the incorporation of a greater proportion of managed plants into tropical forager subsistence systems40. Population recovery during the second half of the mid-Holocene (after 6 k cal BP) is consistent with a florescence linked to a diversified and more stable resource base following climatic stabilisation to a drier yet steady phase7,48,49,50,51,52,53. A possible outcome of these cultural and environmental trajectories was the emergence, in some regions, of population aggregation and social institutions for the coordination and control of previously-unprecedented population densities among South American populations by the Late Holocene26,47,50,51,52,53,54,55.
Understanding mid-Holocene demographic patterns is predicated on our consideration of climatic variability, as well as against the backdrop of developments in cultivation practices. We follow recent research in noting that the scale of anthropic environmental legacies is necessarily linked to relative population over time43,45,56. Our examination of the archaeological and climatic record of South America provides a continental-scale framework for understanding the interplay between population dynamics and food procurement diversification over the mid-Holocene, as well as questioning at what point climate change or instability demands alternative pathways be adopted by human populations. In this regard, the broadening of the trophic niche of humans through the adoption of a greater proportion of plant resources may have functioned as a buffer against environmental unpredictability6,28,29,40. We can generalise that human populations in this period experienced significant and sustained periods of demographic downturn on a continental scale. These demographic processes were not uniform in either time or space and likely encompass substantial variation below the spatial scale we have adopted here. In particular, previously-identified local responses to mid-Holocene climate change require further investigation in the context of our findings.
The demographic signals highlighted on a broad scale in this work are composites of local archaeological records. Statistically significant deviations, whether negative or positive, invite further investigation into the trajectories adopted by human populations at a variety of spatial scales and settings. We anticipate that more research, ideally combining computational vegetation reconstructions and landscape modelling, will help to formally characterise how the demography of South America was shaped by, and in turn shaped, environmental conditions in the long term. Systematic assessments of cultural and biotic resilience to hydroclimatic variability are necessary to understand the development of both domains in the millennia following the human colonisation of South America.
Archaeological radiocarbon analysis
Our analysis employs a database of archaeological radiocarbon determinations compiled from a continuous and ongoing survey of the published academic and grey literature, with an especial focus on Amazonia. Our own collection is cross-referenced to large pre-existing databases5,57 and corrected with reference to the original published sources. Our data collection resulted in a set of 5450 radiocarbon determinations for the interval 12 k – 2 k 14C years before present. In contrast to previous compilations over this period5, we furnish a much larger sample of radiocarbon dates for Amazonia and circum-Amazonia, providing better control over this area.
We make use of the R package ‘rcarbon 1.2’58 to perform statistical analyses on our data. Following established frameworks for the aggregate analysis of archaeological 14C, we examine summed probability distributions (SPDs) of calibrated radiocarbon dates as a proxy for relative change in population over time. This approach to archaeological radiocarbon data rests on the assumption that higher past populations deposit more archaeological material to date, in turn resulting in the production of radiocarbon determinations commensurate with ancient demography21,59,60,61, i.e. an assumption of monotony between dated archaeological charcoal and past population levels. Sampling bias, time-dependent and spatially variable taphonomic loss, laboratory errors, calibration curve fluctuations, and sample contamination can potentially introduce systematic errors that obscure or exacerbate genuine demographic signals in the 14C record. Our mitigation measures for these issues are described in detail in the Supplementary Information. Deletion and loss of archaeological sites is unlikely to have operated on spatiotemporal scale sufficient to bias the record of an entire continent consistently7,8,10,50, and we take our 14C data to be broadly representative despite expectations of some localised site loss.
We initially perform the analysis on the entire South America dataset. However, the global trend may mask significant regional variation in potential subsets of the data. With reference to spatial structure, sites tend to be highly clustered in, for example, the desert coast of Peru, while being diffuse in the central Amazon basin, rendering a single clustering metric inappropriate for discovering viable subdivisions. Formal methods for grouping spatial point patterns such as k-means or density-based clustering are, respectively, unable to adapt to the arbitrary shape of the point pattern and the high variation in the spatial density of sites. For the purposes of the analysis, we choose to partition the South American radiocarbon data into three to investigate human population patterns and capture variation in the structure of the data within broad biogeographical and climatic realms, rather than any specific archaeological cultures or phenomena. The appearance of consistent patterns in the radiocarbon data should therefore reflect robust and independent cross-regional demographic trends. An objective of our study is to consider coeval shifts in demographic and climatic regimes at and around the transition to the Middle Holocene (8.2 k cal BP).
We have opted to divide all sites located above the 300 m elevation contour into northern and southern subsets along the Peru-Chile border, to form the core of the Highlands and Southern Cone datasets. Both sets include Pacific coast sites located to the west of the elevation cut-off point. We assigned dates from Bolivia in La Paz, Oruro, and Cochabamba departments to the Highlands, and those located in Chuquisaca, Tarija, and Potosí to the Southern Cone. The remainder of the Southern Cone consists of dates from Uruguay, Argentina, Paraguay, and the Brazilian states of Paraná, Rio Grande do Sul, and Santa Catarina. Sites located below 300 m above sea level and outside of the abovementioned elevation boundaries form the Lowlands dataset. These criteria produce three subsets of the data that group lowland Amazonia with the Guianas, the Orinoco basin, and northeast Brazil (here the Tropical Lowlands), the northern Pacific coast and Andes with the Amazonian foreland basin and foothills (here the Tropical Highlands), and finally the southern Pacific coast and Andes with the Pampas, Patagonia and the southern Brazilian highlands (the Southern Cone). With reference to the climatic systems that drive rainfall variability across South America (Supplementary Information), our subdivisions correspond approximately (in order) to areas principally influenced by moisture transportation from the Atlantic, areas influenced by the Atlantic and potentially Pacific sources, and predominantly South Pacific/Atlantic-influenced zones13,23,24,25. The Southern Cone also functions as a geographical proxy for the southernmost range of tropical domesticates before the late Holocene6,26.
The statistical modelling presented here principally concerns the mid-Holocene, but we carry out our analyses on dates in the interval 12–2 k cal BP. Dates pre-dating 12 k 14C years BP were excluded, as were those with Gaussian errors of >200 years. Although the initial peopling of South America was underway by 14 k cal BP, and possibly earlier4, the sparse evidence available for this period and its distance from the mid-Holocene makes it less germane to this study. Our initial analyses on the 5450 radiocarbon determinations acquired for the entire continent employ the following protocol:
Calibration. Radiocarbon dates are calibrated and aggregated by site into non-overlapping phases over the period 12–2 k 14C BP, and we report on the results from a focused time range of 9 k – 3 k cal BP. Aggregation of dates from the same site into bins of 200 years is carried out to account for the overrepresentation of well-dated sites. We principally make use of the SHCal13 curve62 for calibration (Fig. S4), except for determinations on marine shells, for which the Marine13 curve63 is used. This calibration curve is offset from the terrestrial curves by several centuries to account for the incorporation of ancient carbon from ocean upwelling into mollusc exoskeletons. In addition, we calibrate marine dates using local averaged ΔR offsets and errors by interpolating to the geographically-closest sampling site, acquired through an online repository64.
Summation. The probability distributions of the calibrated dates are summed for the entire South American continent. We do not normalise the post-calibration probability distributions, to reduce the effect of peaks and plateaus in the calibration curves on the shape of the final SPDs19 (Sensitivity Analysis).
Model testing. We initially test the South America-wide SPD against a simple fitted exponential population growth trend. Our choice is guided by an information criterion indicating maximum goodness-of-fit with this model over linear and logistic growth models (Sensitivity Analysis). A sample of calendar dates equal to the number of bins are drawn from the fitted model, converted to 14C dates, re-calibrated, and their probability distributions summed. We opt to draw from the uncalibrated date distribution. Errors for the re-calibration were generated by sampling with replacement from the empirical 14C errors of both marine and terrestrial dates58. Through a Monte Carlo procedure of 1000 runs, we derive confidence intervals for the null model.
Regional permutation tests. Through the random assignation of marks to each date in the complete dataset, in this case the regional affiliation, a distribution of simulated SPDs are generated from 1000 Monte Carlo runs27, from which 95% confidence intervals are derived. The empirical SPDs were directly compared with the pan-regional trend produced from permuting the marks of the full dataset (n = 5450). We also performed pairwise comparisons of each of the three regions (Fig. S2).
The above procedures controls for the global effects of taphonomy and first-order spatial processes such as sea level rise, as exponential model fitting mimics the effects of time-dependent loss and the permutation tests makes use of the 14C determinations directly, in effect integrating the effects of taphonomic loss into the analysis27,65. Our initial results with this exploratory null model suggest statistically significant relative population decline during the Middle Holocene. Nonetheless, the initial formulation of the null hypothesis may be responsible for this finding (a Type I error) and thus we extend our model testing further to resolve this issue.
We follow Silva and Vanderlinden22 in specifying a null model based on prevailing patterns prior to a target period of interest rather than the dataset as a whole. Through inspection of our exponential model (Fig. 1), we first note that this null hypothesis performs particularly poorly in the Terminal Pleistocene and Early Holocene, and second, the SPD suggests a slow decline in the South American 14C record may begin already around 9 k cal BP. Further testing (Supplementary Information) indicates that the point of divergence from approximately stable, weakly linear Early Holocene demographic regimes5 is most likely to have occurred at 8.6 k cal BP (Fig. S1), providing a point of departure for investigating the degree to which mid-Holocene demographic trends diverge from prior patterns. That is, instead of a single null hypothesis for 12–2 k cal BP, we condition our new null model on the period 12–8.6 k cal BP to satisfactorily identify the degree of deviation after this point in time.
Our protocols allow us to examine salient features of the South American radiocarbon record, specifically: (a) the degree and significance of deviation in demographic trends from the null hypothesis of steadily increasing population throughout the Early Holocene in our domain, and (b) the similarity of population trajectories in each regional setting. Both sets of tests permit local and global tests of significance to be estimated, and regional population histories to be compared through z-transformation of the empirical and simulated SPDs58. We plot the null models against the empirical SPDs with a running average over a century applied to smooth out artefacts of the calibration process in the probability distributions.
Climate variability index
To our knowledge there is no single prevailing criterion for defining a climatic anomaly, with a variety of thresholds, methods, and selection procedures reported in the literature66. To derive a robust index of climate variability we use the Median Absolute Deviation (MAD), a measure of statistical dispersion, on a set of precipitation records with near-complete latitudinal coverage of South America (Fig. S3). MAD provides a symmetrical estimate of the central tendencies of a time series, and effectively accounts for the value of any given data point in the context of a rolling window. It is not sensitive to isolated extreme outliers or non-normal distributions, and therefore correctly identifies sudden, large-amplitude changes and oscillations while excluding general trends. Here, we impose ±3 times the rolling MAD as a conservative threshold for identifying an extreme outlier. We scale the window to the resolution of each individual palaeoclimatic record to approximate a 100-year rolling average and have selected records with a resolution sufficient to allow a minimum of three points per interval. Where records have multiple sources of data, for example two speleothems reported from Lapa Grande cave, Brazil, the outlier analysis has been performed separately on these sources and subsequently combined.
We sum the identified anomalies from each record into 100-year bins to present them on a common time scale of calendar years before present (cal BP) and produce an intuitive summary index of climatic outliers for continental South America. In addition to the high initial MAD threshold for defining an abrupt event in each individual record, we suggest that frequencies of binned anomalies that are two standard deviations over the dataset mean are indicative of significantly above-normal climatic variability. Finally, we underline that the results simply reflect the sum of anomalies in a given time step, not their magnitude, spatial distribution, or whether they are negative or positive deviations from the rolling MAD of a given precipitation record. Deriving a composite measure of climatic variability aims to contextualise our demographic data (Figs 2 and 3) rather than directly infer the state of the climate or environment itself. We rely on the interpretations palaeoclimatologists and palaeoecologists to understand the conditions and impacts of climate on humans in our modelling domain.
Brenner, M. et al. Abrupt climate change and pre-Columbian cultural collapse. In: Markgraf, V. (ed.), Interhemispheric climate linkages 87–103 (2001).
Meltzer, D. J. First Peoples in a New World: Colonizing Ice Age America. Berkeley: University of California Press (2009).
Moore, J. D. A Prehistory of South America. Boulder: University Press of Colorado (2014).
Dillehay, T. D. et al. New archaeological evidence for an early human presence at Monte Verde, Chile. PLoS One 10, e0141923 (2015).
Goldberg, A., Mychajliw, A. M. & Hadly, E. A. Post-invasion demography of prehistoric humans in South America. Nature 532, 232–235 (2016).
Perez, S. I., Postillone, M. B. & Rindel, D. Domestication and human demographic history in South America. Am. J. Phys. Anthropol. 163, 44–52 (2017).
Neves, E. G. El Formativo que nunca terminó: la larga historia de estabilidad en las ocupaciones humanas de la Amazonía central. Boletín de Arqueología PuCP 11, 117–142 (2007).
Grosjean, M. et al. Mid-Holocene climate and culture change in the South Central Andes. In: Anderson, D. G., Maasch, K. & Sandweiss, D. H. (eds), Climate Change and Cultural Dynamics: A Global Perspective on Mid-Holocene Transitions. Cambridge, MA: Academic Press. 51–115 (2011).
Barberena, R., Méndez, C. & de Porras, M. E. Zooming out from archaeological discontinuities: The meaning of mid-Holocene temporal troughs in South American deserts. J. Anthropol. Archaeol. 46, 68–81 (2017).
Araujo, A. G. M. et al. Holocene dryness and human occupation in Brazil during the “Archaic Gap”. Quaternary Research 64, 298–307 (2005).
Burbano, M. E. D. Mid and Late Holocene population changes at the Sabana de Bogotá (Northern South America) inferred from skeletal morphology and radiocarbon chronology. Quaternary International 256, 2–11 (2012).
Núñez, L., Grosjean, M. & Cartajena, I. Human occupations and climate change in the Puna de Atacama, Chile. Science 298, 821–824 (2002).
Walker, M. J. C. et al. Formal subdivision of the Holocene Series/Epoch: a Discussion Paper by a Working Group of INTIMATE. J. Quat. Sci 27, 649–659 (2012).
Deininger, M. et al. Late Quaternary Variations in the South American Monsoon System as Inferred by Speleothems—New Perspectives using the SISAL Database. Quaternary 2, 6 (2019).
Riede, F. Climate and demography in early prehistory: using calibrated 14C dates as population proxies. Human Biology 81, 309–38 (2009).
Anderson, D. G., Maasch, K. & Sandweiss, D. H. (eds), Climate Change and Cultural Dynamics: A Global Perspective on Mid-Holocene Transitions. Cambridge, MA: Academic Press. (2011).
Kelly, R. L. et al. A continuous climatic impact on Holocene human population in the Rocky Mountains. Proc. Natl Acad. Sci. USA 110, 443–7 (2013).
Warden, L. et al. Climate induced human demographic and cultural change in northern Europe during the mid-Holocene. Scientific Reports 7, 15251 (2017).
Bevan, A. et al. Holocene fluctuations in human population demonstrate repeated links to food production and climate. Proc. Natl Acad. Sci. USA 114, E10524–E10531 (2017).
Bronk Ramsey, C. Methods for summarizing radiocarbon datasets. Radiocarbon 59, 1809–1833 (2017).
Shennan, S. et al. Regional population collapse followed initial agriculture booms in mid-Holocene Europe. Nat Commun 4, 2486 (2013).
Silva, F. & Vanderlinden, M. Amplitude of travelling front as inferred from 14C predicts levels of genetic admixture among European early farmers. Scientific Reports 7, 11985 (2017).
Deplazes, G. et al. Links between tropical rainfall and North Atlantic climate during the last glacial period. Nature Geoscience 6, 213–217 (2013).
Cruz, F. W. et al. Orbitally driven east–west antiphasing of South American precipitation. Nature Geoscience 2, 210–214 (2009).
Schneider, T., Bischoff, T. & Haug, G. H. Migrations and dynamics of the intertropical convergence zone. Nature 513, 45–53 (2014).
Iriarte, J. et al. Evidence for cultivar adoption and emerging complexity during the mid-Holocene in the La Plata basin. Nature 432, 614–617 (2004).
Crema, E. R. et al. Summed probability distribution of 14C dates suggests regional divergences in the population dynamics of the Jomon period in eastern Japan. PLoS One 11, e0154809 (2016).
Morgan, C. Climate change, uncertainty and prehistoric hunter–gatherer mobility. J. Anthropol. Archaeol. 28, 382–396 (2009).
Sandweiss, D. H. et al. Environmental change and economic development in coastal Peru between 5,800 and 3,600 years ago. Proc. Natl Acad. Sci. USA 106, 1359–1363 (2009).
Smith, R. J. & Mayle, F. E. Impact of mid-to late Holocene precipitation changes on vegetation across lowland tropical South America: a paleo-data synthesis. Quaternary Research 89, 134–155 (2018).
Haberzettl, T. et al. Late glacial and Holocene wet–dry cycles in southern Patagonia: chronology, sedimentology and geochemistry of a lacustrine record from Laguna Potrok Aike, Argentina. The Holocene 17, 297–310 (2007).
Rein, B. et al. El Niño variability off Peru during the last 20,000 years. Paleoceanography and Paleoclimatology 20, PA4003 (2005).
Marchant, R. et al. Pollen-based biome reconstructions for Latin America at 0, 6000 and 18 000 radiocarbon years ago. Clim. Past 5, 725–767 (2009).
Urrego, D. H. et al. Millennial-scale ecological changes in tropical South America since the last glacial maximum. In: Vimeux, F., Florence, S. & Khodri, M. (eds) Past climate variability in South America and surrounding regions. 283–300 Dordrecht: Springer (2009).
Stevens, C. J. & Fuller, D. Q. Alternative strategies to agriculture: the evidence for climatic shocks and cereal declines during the British Neolithic and Bronze Age (a reply to Bishop). World Archaeology 47, 856–875 (2015).
Mayle, F. E. & Power, M. J. Impact of a drier Early–Mid-Holocene climate upon Amazonian forests. Proc. R. Soc. B 363, 1829–1838 (2008).
Piperno, D. R. & Pearsall, D. M. The origins of agriculture in the lowland Neotropics. Cambridge, MA: Academic Press (1998).
Gnecco, C. Against ecological reductionism: Late Pleistocene hunter-gatherers in the tropical forests of northern South America. Quaternary International 109, 13–21 (2003).
Clement, C. R. et al. The domestication of Amazonia before European conquest. Proc. R. Soc. B 282, 20150813 (2015).
Piperno, D. R. The origins of plant cultivation and domestication in the New World tropics: patterns, process, and new developments. Current Anthropology 52, S453–470 (2011).
Piperno, D. R. & Dillehay, T. D. Starch grains on human teeth reveal early broad crop diet in northern Peru. Proc. Natl Acad. Sci. USA 105, 19622–19627 (2008).
Hilbert, L. et al. Evidence for mid-Holocene rice domestication in the Americas. Nature Ecology &. Evolution 1, 1693–1698 (2017).
Larson, G. et al. Current perspectives and the future of domestication studies. Proc. Natl Acad. Sci. USA 111, 6139–6146 (2014).
Arroyo-Kalin, M. Slash-burn-and-churn: Landscape history and crop cultivation in pre-Columbian Amazonia. Quaternary International 249, 4–18 (2012).
Boivin, N. L. et al. Ecological consequences of human niche construction: Examining long-term anthropogenic shaping of global species distributions. Proc. Natl Acad. Sci. USA 113, 6388–6396 (2016).
Roberts, P. et al. The deep human prehistory of global tropical forests and its relevance for modern conservation. Nature Plants 3, 17093 (2017).
Brooks, N. Cultural responses to aridity in the Middle Holocene and increased social complexity. Quaternary International 151, 29–49 (2006).
Roosevelt, A. C. et al. Eighth millennium pottery from a prehistoric shell midden in the Brazilian Amazon. Science 254, 1621–1624 (1991).
Sandweiss, D. H. Terminal Pleistocene through Mid-Holocene archaeological sites as paleoclimatic archives for the Peruvian coast. Palaeogeography, Palaeoclimatology, Palaeoecology 194, 23–40 (2003).
Lombardo, U. et al. Early and middle Holocene hunter-gatherer occupations in Western Amazonia: the hidden shell middens. PLoS One 8, e72746 (2013).
Villagran, X. S. & Giannini, P. C. F. Shell mounds as environmental proxies on the southern coast of Brazil. The Holocene 24, 1009–1016 (2014).
Yacobaccio, H. D., Morales, M. R. & Hoguin, R. Habitats of ancient hunter-gatherers in the Puna: Resilience and discontinuities during the Holocene. J. Anthropol. Archaeol. 46, 92–100 (2017).
Watling, J. et al. Direct archaeological evidence for Southwestern Amazonia as an early plant domestication and food production centre. Plos One 13, e0199868 (2018).
Sandweiss, D. H., Maasch, K. A. & Anderson, D. G. Transitions in the mid-Holocene. Science 283, 499–500 (1999).
Pozorski, T. & Pozorski, S. Early Complex Society on the North and Central Peruvian Coast: New Archaeological Discoveries and New Insights. J. Arch. Research, 1–34 (2017).
Arroyo-Kalin, M. Landscaping, Landscape Legacies, and Landesque Capital in Pre-Columbian Amazonia. In: Isendahl, C. & Stump, D. (eds), The Oxford Handbook of Historical Ecology and Applied Archaeology. 1–24 Oxford: OUP (2016).
Bueno, L. et al. A Late Pleistocene/early Holocene archaeological 14C database for South America and the Isthmus of Panama: Palaeoenvironmental contexts and demographic interpretations. Quaternary International 301, 1–2 (2013).
Bevan, A. & Crema, E. R. rcarbon v1.2: Methods for calibrating and analysing radiocarbon dates. URL: https://CRAN.R-project.org/package=rcarbon (2018).
Timpson, A. et al. Reconstructing regional population fluctuations in the European Neolithic using radiocarbon dates: a new case-study using an improved method. J. Arch. Sci. 52, 549–557 (2014).
Rick, J. W. Dates as data: an examination of the Peruvian preceramic radiocarbon record. American Antiquity 52, 55–73 (1987).
Williams, A. N. The use of summed radiocarbon probability distributions in archaeology: a review of methods. J. Arch. Sci. 39, 578–589 (2012).
Hogg, A. G. et al. SHCal13 Southern Hemisphere calibration, 0–50,000 years cal BP. Radiocarbon 55, 1889–1903 (2013).
Reimer, P. J. et al. IntCal13 and Marine13 radiocarbon age calibration curves 0–50,000 years cal BP. Radiocarbon 55, 1869–1887 (2013).
Reimer, R. 14CHRONO Marine Reservoir Database., http://calib.org/marine/ (2018).
Surovell, T. A. & Brantingham, P. J. A note on the use of temporal frequency distributions in studies of prehistoric demography. J. Arch. Sci. 34, 1868–1877 (2007).
Kintigh, K. & Ingram, S. E. Was the drought really responsible? Assessing statistical relationships between climate extremes and cultural transitions. J. Arch. Sci 89, 25–31 (2018).
Data collection was partially funded by a UCL CREDOC Small Grant awarded to M.A.K. P.R. was funded by a Visiting Fellowship at the Sainsburys Research Unit, Sainsburys Centre for Visual Arts, University of East Anglia and a British Academy Postdoctoral Fellowship (PF2\180065) at UCL. The authors acknowledge the help of Fabio Silva in developing elements of the methods reported on here.
The authors declare no competing interests.
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Riris, P., Arroyo-Kalin, M. Widespread population decline in South America correlates with mid-Holocene climate change. Sci Rep 9, 6850 (2019). https://doi.org/10.1038/s41598-019-43086-w
Sedimentary unknowns constrain the current use of frequency analysis of radiocarbon data sets in forming regional models of demographic change
Delayed demographic transition following the adoption of cultivated plants in the eastern La Plata Basin and Atlantic coast, South America
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Did pre-Columbian populations of the Amazonian biome reach carrying capacity during the Late Holocene?
Philosophical Transactions of the Royal Society B: Biological Sciences (2021)
Philosophical Transactions of the Royal Society B: Biological Sciences (2021)