Table 2 Subgroup analysis of isolation outcome parameters.

From: Impact of Percoll purification on isolation of primary human hepatocytes

Parameter Overall high initial viability > 80% low initial viability <80%
Mean ± SEM p-Value Mean ± SEM p-Value Mean ± SEM p-Value
Percoll− Percoll+ Percoll− Percoll+ Percoll− Percoll+
Plating efficiency (%) 27.19 ± 2.19 31.77 ± 2.99 0.0455 29.92 ± 1.87 34.33 ± 3.05 0.0783 11.735 ± 2.47 16.46 ± 3.48 0.4312
Viablility (%) 84.47 ± 1.60 88.18 ± 1.13 0.0299 87.39 ± 0.91 88.29 ± 1.27 0.3209 71.63 ± 4.44 87.70 ± 2.79 0.0625
Percoll survival (%) 59.03 ± 3.90    59.63 ± 4.10    56.38 ± 12.02   
Relative yield in millions per gram liver tissue 42.40 ± 4.60 26.60 ± 4.00 <0.0001 46.15 ± 4.95 29.18 ± 4.40 <0.0001 25.90 ± 9.72 15.30 ± 8.66 0.0299
Total protein on culture plate on day 6 596.20 ± 66.79 662.70 ± 61.98 0.0102 643.76 ± 73.55 716.71 ± 66.50 0.0156 334.38 ± 79.79 365.58 ± 55.33 0.6250