Carbon Catabolite Repression in Yeast is Not Limited to Glucose

Cells adapt their gene expression and their metabolism in response to a changing environment. Glucose represses expression of genes involved in the catabolism of other carbon sources in a process known as (carbon) catabolite repression. However, the relationships between “poor” carbon sources is less characterized. Here we show that in addition to the well-characterized glucose (and galactose) repression of ADH2 (alcohol dehydrogenase 2, required for efficient utilization of ethanol as a carbon source), ADH2 expression is also inhibited by acetate which is produced during ethanol catabolism. Thus, repressive regulation of gene expression occurs also between “poor” carbon sources. Acetate repression of ADH2 expression is via Haa1, independently from the well-characterized mechanism of AMPK (Snf1) activation of Adr1. The response to extracellular acetate is attenuated when all three acetate transporters (Ady2, Fps1 and Jen1) are deleted, but these deletions do not affect the acetate response resulting from growth with glucose or ethanol as the carbon source. Furthermore, genetic manipulation of the ethanol catabolic pathway affects this response. Together, our results show that acetate is sensed intracellularly and that a hierarchical control of carbon sources exists even for “poor” carbon sources.

cerevisiae genome. Cells expressing only ADH1 produce ethanol during fermentation of glucose comparably to wild-type cells, and can metabolise ethanol 34 Adh2 has a 20-fold higher affinity for ethanol than Adh1 35 and thus cells expressing only ADH2 do not produce ethanol during glucose fermentation, but are capable of ethanol catabolism 34 . The mitochondrial Adh3 is expressed at low levels and forms the mitochondrial component of the ethanol-acetyladehyde shuttle to regenerate mitochondrial NAD from NADH 36 . However, cells expressing only ADH3 are capable to both produce and utilize ethanol (similarly to ADH1). Cells expressing solely ADH4 or ADH5 are incapable of producing or metabolizing ethanol 34 . Acetylaldehyde is oxidized to acetic acid primarily by the cytoplasmic Ald6 and the mitochondrial Ald4 and Ald5 enzymes; the first two use NADP as a co-factor, whereas the latter utilizes NAD 37,38 . Acetate is then conjugated to coenzyme-A in the cytoplasm by two acetyl-coA synthetases: Acs1, which is glucose-repressed 39 and Acs2, which is constitutively expressed 40 . Cytoplasmic Acetyl-coA has important roles in fatty acid synthesis 41 , acetylation of proteins such as histones 42 , biosynthesis of sterols and amino acids, and for entry into the Krebs' cycle via the glyoxylate pathway, which is repressed in the presence of glucose 43 . A simplified diagram of metabolic pathways pertaining to ethanol and acetate metabolism is presented in Fig. S1.
Despite the central role played by acetate in yeast metabolism, the location of acetic acid sensing has not been yet determined. Here we demonstrate that acetate, a product of both fermentation and ethanol catabolism and itself a carbon source, inhibits expression of genes involved in ethanol catabolism. We show that acetate is sensed intracellularly, and a consequence of this is that ethanol metabolism results in the induction of the acetate response. Our results uncover the existence of catabolite repression among sugars that are considered "poor" carbon sources.

Acetate represses expression of genes involved in ethanol metabolism. Expression of the ADH2
gene, encoding alcohol dehydrogenase, is tightly regulated by carbon source, being low in the presence of glucose, and high on poor carbon sources. Although the inhibition of ADH2 expression by glucose via the inactivation of Snf1 has been well-characterized (Snf1 is needed to activate the Adr1 and Cat8 transcription factors) 44 , it is still unclear whether ADH2 expression requires a positive signal from the poor carbon source. We therefore examined ADH2 expression in response to a range of carbon sources, by measuring an increase in β-galactosidase activity following transfer of cells bearing an prADH2::LacZ reporter from 4% glucose medium to media containing other carbon sources. As previously shown 45 , ADH2 was repressed on medium containing glucose. Whereas earlier work demonstrated that abolition of glucose repression results in constitutive ADH2 expression 46 , and that ADH2 is induced by a variety of carbon sources including those whose metabolism does not involve Adh2 47 , we observed that even in the absence of any carbon source ADH2 is highly expressed (Fig. 1a), suggesting a lack of any positive signaling mechanism. However, we consistently observed a repression of ADH2 expression by 2% acetate (pH 6). ADH2 expression in acetate was very low, and acetate repressed ADH2 expression in the presence of glycerol or ethanol (Fig. 1a). Even prolonged growth of yeast (24 h) in medium containing acetate (pH6) did not result in medium acidification, suggesting that the observed effects of acetate are not due to the acidity itself, but rather by the acetate moiety. It is possible, however, that the elevated pH of this medium (pH6) results in the abrogation of ADH2 expression. However, although ADH2 expression is slightly attenuated when cells are grown in phosphate buffered medium (pH6) containing 2% ethanol, the acetate repression of ADH2 expression is far more severe, indicating that this is not due to pH. Oleic acid catabolism via beta-oxidation proceeds directly to acetyl-coA and does not produce acetate. Oleic acid (0.14%) did not cause repression of ADH2 expression, suggesting that this repression is specific to acetate and not to acetyl-coA or to other poor carbon sources.
We examined the level of acetate-repression of ADH2 expression upon transfer of cells from glucose to ethanol containing different concentrations of acetate. ADH2 expression started to be reduced at 0.25% acetate, with more severe repression at higher concentrations (Fig. 1b). Since different genetic backgrounds of S. cerevisiae contain different mutations (in the "wild-type") and this may affect respiration 48 , we examined the effects of increasing acetate concentrations on ADH2 expression additionally in the W303-1b background (HAP1) and in the V5 champagne yeast background 38 . W303-1b was indistinguishable from BY4741. ADH2 repression by acetate was slightly reduced in the V5 background, but a marked lowering of ADH2 expression still occurred (Fig. 1b). This suggest that the effects of acetate upon ADH2 expression is not limited to the BY4741 yeast background.
The transcription factor Haa1 mediates the response to acetate stress in glucose-grown cells; it is responsible for the increased expression of genes in the acetate response, such as YRO2 and TPO3 28,29,49 . Inspection of the ADH2 promoter showed that a potential Haa1 binding site is found at −343 before the ORF's ATG. We found that deletion of HAA1 dramatically enhances ADH2 expression in ethanol and derepresses ADH2 expression in acetate (Fig. 1c).
Hrr25 regulates ADH2 expression. Phosphorylated Haa1 is exported from the nucleus in an Msn5 dependent manner 50 , and recently the casein kinase Hrr25 has been shown to phosphorylate Haa1 and inhibit its activity, with deletion of HRR25 resulting in increased expression of the acetate-induced gene YRO2 and retention of Haa1 in the nucleus even in the absence of acetate 51 . We examined ADH2 expression in hrr25 strains. Expression of ADH2 was attenuated in ethanol in both Δhrr25 and hrr25-E52D mutants 51 . This effect was due to Haa1 hyperactivation, as deletion of HAA1 restored ADH2 expression (Fig. 2a). To demonstrate the centrality of Haa1 we deleted the karyopherin-encoding gene MSN5. This results in an increased nuclear retention of Haa1 50 , and, as expected, in a reduction in the expression of ADH2 in ethanol. Again, this repression could be reverted by deletion of HAA1 (Fig. 2b).
The most studied mechanism of ADH2 expression regulation is AMPK-Snf1 activation of the Adr1 transcription factor 44 . However, deletion of HAA1 did not compensate for lack of Adr1 (Fig. 2c), nor did hyperactive Adr1 S230A 44 restore ADH2 expression in acetate media, or in Δhrr25 cells in ethanol media (Fig. 2d). We therefore www.nature.com/scientificreports www.nature.com/scientificreports/ conclude that the Adr1 and Haa1 pathways that regulate ADH2 expression seem to be independent from each other.
Location of Acetate sensing. Anionic acetate is imported into the cell by the Jen1 and Ady2 transporters, and at low pH (below 4.76) undissociated acetic acid undergoes facilitated diffusion by Fps1 (reviewed in 21 ). Acetate, being a small molecule can also passively cross the plasma membrane 32 . However, whether acetate is sensed inside or outside the cell has not been established. We generated double and triple knockouts of the acetate importers, and found that ADH2 expression was restored to 50% of its ethanol expression levels when cells were grown in acetate media upon deletion of all three importers (Ady2, Fps1 and Jen1 -ΔΔΔafj) (Fig. 3a). To confirm that this is due to acetate, we deleted ACS1, which converts acetate to acetyl-coA ( Fig. S1) in order to lower the flux from acetate to acetyl-coA. Deletion of ACS1 lowered ADH2 expression and suppressed the high ADH2 expression (in acetate) phenotype of ΔΔΔafjcells (Fig. 3a). We wondered whether the changes of ADH2 expression affect ADH activity for the oxidation of alcohols. We performed ADH assays on yeast extracts from cells grown with different carbon sources. The assay does not discriminate between different ADH enzymes, and so glucose-grown cells have an ADH activity of 20 mU/OD which is present in Δadh2 cells (and is thus due to the other ADH enzymes, primarily Adh1). ADH enzyme activity correlates with ADH2 gene expression above this threshold in ethanol and acetate grown cells, with Δhaa1 and ΔΔΔafj cells having much higher ADH activity in ethanol and acetate conditions which is reversed by additional deletion of ACS1. Interestingly growth with oleate as the sole carbon source results in abrogation of all ADH activity, suggesting post-transcriptional regulation is occurring. Expression of YRO2 was reduced by 50% in the ΔΔΔafj strain) when acetate was added to cells for a 3 hour period (Fig. 3c). We examined the expression of YRO2 in cells grown with differing concentrations of acetate and other carbon sources. Ethanol catabolism produces intracellular acetate, and this was sufficient to induce an acetate response (Fig. 3d). In contrast, oleic acid catabolism does not produce acetate, and YRO2 was not expressed upon growth with oleic acid as the sole carbon source. For determining the effect of acetate concentration upon YRO2 expression, experiments were carried out under respiratory conditions with glycerol as the www.nature.com/scientificreports www.nature.com/scientificreports/ carbon source, since glycerol metabolism does not involve ADH2. For both BY4741a and W303-1b backgrounds we observed a progressive increase in YRO2 expression with increasing acetate concentration, though high levels of YR02 expression occur only at 1% or higher concentrations of acetate (Fig. 3d). We note that these acetate concentrations are higher than previously reported for YRO2 expresssion 51 since most experiments are carried out in the presence of glucose, which causes acute acetic acid stress 21 . Similarly to ADH2 repression (Fig. 1d), the V5 background exhibits an attenuated response to acetate.
To further confirm the importance of the acetate transporters for the acetate genetic response, we examined Haa1-GFP localization in glucose and following addition of acetate (pH 6) to 2% for 45 minutes. As previously reported, Haa1-GFP is constitutively nuclear in Δhrr25 cells 51 . Whereas a strong nuclear Haa1-GFP localization is present in WT cells 45 minutes after addition of acetate to 2%, Haa1-GFP did not localize to the nucleus in ΔΔΔafj cells (Fig. 3d,e).
Since ethanol catabolism produces acetate, we reasoned that measuring YRO2 expression (acetate-induced) in cells growing on ethanol versus acetate may provide a reasonable proxy from endogenous versus exogenous acetate. We grew cells over a 24 hour period and measured YRO2 expression (Fig. 3g). Glucose induces about 15% of YRO2 expression compared to acetate, presumably due to conversion of acetylaldehyde to acetate during fermentation. Likewise, ethanol induces 40% of the YRO2 expression seen in acetate. These results imply that conversion of ethanol to acetate is faster than utilization of acetate and that acetate can function as a carbon reserve for cells. In contrast, oleic acid does not induce YRO2 expression. Whereas YRO2 expression was reduced by 70% in the ΔΔΔafj strain during growth on acetate, expression was unaffected when cells were grown on glucose or ethanol, strongly suggesting that acetate is indeed sensed intra-celullarly. Upon deletion of ACS1 in ΔΔΔafj cells (thus restricting conversion of acetate to acetyl-coA), YRO2 expression was somewhat increased when cells were grown on acetate (though not to the same extent as in Δacs1 cells). Furthermore, deletion of ACS1 also increased YRO2 expression in glucose and ethanol grown cells, presumably due to increased acetate concentration in the cell (Fig. 3h).
other ethanol metabolic genes are also acetate-repressed. We analyzed expression of the other genes in the ethanol to acetyl-coA pathway (Fig. S1) upon growth on glucose, ethanol, acetate or oleic acid as the sole carbon source. Oleic acid was included as its catabolism provides an alternate source of cytoplasmic acetyl-coA, but does not induce Haa1-mediated transcriptional regulation (which responds to short chain organic acids such as acetic, propionic and lactic acids) 49 . Whereas ALD5 expression was unchanged by deletion of HAA1, deletion of HAA1 resulted in modest increases of ALD4 and ALD6 expression in acetate and oleic acid media, suggesting that Haa1 contributes to repression of these genes when cells can generate cytoplasmic acetyl-coA (Fig. S2a-c). ACS1 expression was glucose-repressed as previously reported 39 and expression in acetate was partially lowered in Δhaa1 cells (Fig. S2d). ACS2 expression was strongest in ethanol. No effect of Haa1 could be discerned (Fig. S2e). Thus, it seems that although Haa1 does regulate other genes in the ethanol-acetyl-coA pathway to promote a lowering of acetate levels, the major regulation that Haa1 exerts is at the ADH2 level. www.nature.com/scientificreports www.nature.com/scientificreports/ Metabolic engineering of the acetate response. If acetate synthesized intracellularly by ethanol catabolism can be sensed to cause YRO2 expression, then we would expect genetic manipulation of this pathway to exert effects upon gene expression (Fig. 4). Indeed, overexpression of ALD6 results in a dramatic increase in YRO2 expression when cells are grown on glucose or ethanol, but not when grown on acetate or oleate, suggestive of increased acetate production from acetyladehyde. Similarly, YRO2 expression was reduced in glucoseor ethanol-containing media in Δald4 Δald6 cells, which lack two of the five redundant enzymes that convert acetylaldehyde into acetate (YRO2 expression in response to acetate was unchanged in Δald4Δald6 cells.) YRO2 expression in glucose or ethanol was restored in Δald4Δald6Δacs1 cells (Fig. 5a). We compared the contribution of ALD4, ALD5 and ALD6. Single deletions alone did not affect YRO2 expression, nor did the Δald4Δald5 double deletion. Deletion of ALD6 in combination with either Δald4 or Δald5 dramatically reduced YRO2 expression in glucose or ethanol media, and a triple Δald4Δald5Δald6 strain eliminates YRO2 expression to the background levels observed in oleic acid (Fig. 5b).  Fig. 2 (b). Cells were grown overnight in the indicated media (without dilution). 0.8OD was harvested and ADH activity determined. Activity is in mU/OD 600 . N = 3. Error bars are one standard deviation. (c) Cells [WT or Δady2Δfps1Δjen1] were grown overnight in 4% glucose. In the morning, cells were diluted and grown for an additional 3 hours in 4% glucose media. After measuring basal YRO2 expression, sodium acetate ph5.6 was added to 2% and YRO2 expression determined after 3 hours. Rate of expression is normalized against WT cells in acetate media. N = 3. Error bars are one standard deviation. (c) Cells were grown for 24 hours in 5 ml of the indicated media (without dilution) and YRO2 expression measured. Expression is normalized against BY4741 in glycerol +2% acetate medium. N = 3. Error bars are one standard deviation. (d) Cells were grown as in (b) Cells were imaged in 4% glucose media and 45 minutes following addition of acetate (pH 5.6) to 2%. (e) Quantification of d as described in Material and Methods. (f,g) Cells were grown for 24 hours in 5 ml of the indicated media (without dilution) and YRO2 expression measured. Expression is normalized against WT in acetate medium. N = 3. Error bars are one standard deviation. (2019) 9:6491 | https://doi.org/10.1038/s41598-019-43032-w www.nature.com/scientificreports www.nature.com/scientificreports/ Mutations that increase acetate production (ALD6 overexpression) or reduce its consumption (Δacs1) lower ADH2 expression levels (Fig. 5c). ADH2 expression was elevated in Δald6 cells when cells were grown on 2% ethanol (Fig. 5d), but progressively declined upon further deletion of ALD genes. As expected, ADH2 expression remained low when cells were grown in acetate medium. We suspect that this is due to another layer of regulation of ADH2 expression by the very toxic intermediate 52 acetylaldehyde induced by the restriction of acetyladehyde outflow to acetate.

Discussion
Carbon catabolite repression is often considered to be a matter of "good" carbon sources, such as glucose and fructose, actively preventing the metabolism of other carbon sources, and has been much investigated. However, the relationships between poor carbon sources has been of scant enquiry, and thus it was unclear whether similar inter-sugar relationships exist for them, similarly to the relation between glucose and other sugars. Here we have shown that part of the acetate response involves downregulating of ADH2 expression. We also show that there is no positive signal needed to promote ADH2 expression; rather, its expression is negatively regulated by glucose (via Snf1 inhibition of Adr1) 44 , by galactose 53 and its downstream product acetate via the Haa1 transcription factor acting as a repressor (Fig. 4). Similarly to glucose, acetate represses ADH2 expression even when other carbon  (a,b) Cells were grown for 24 hours in 5 ml of the indicated media (without dilution) and YRO2 expression measured. Expression is normalized against WT in acetate medium. N = 3. Error bars are one standard deviation. (c,d) Cells were grown overnight in 4% glucose. In the morning, cells were diluted and grown for an additional 3 hours in 4% glucose media. After measuring the basal ADH2 expression (t = 0), cells were washed three times with DDW and resuspended in media containing either ethanol (2%) or sodium acetate pH6 (2%). ADH2 expression was determined after 3 hours. Rate of expression is normalized against WT cells in ethanol media. N = 3. Error bars are one standard deviation.
www.nature.com/scientificreports www.nature.com/scientificreports/ sources are present. Although gene expression in response to acetate has been previously investigated 28,29 , this level of regulation has been overlooked since glucose independently inhibits ADH2 expression. In addition, most studies involving acetate provide acetic acid as a non-metabolizable (due to glucose) stressor (pH 4), with both acid and acetate contributing to the stress response. Here we have provided dissociated acetate (pH6) as the sole carbon source. Extracellular acetate (pH 6) or intracellularly produced acetate produced via catabolism of glucose or ethanol provoked the Haa1 dependent response previously demonstrated for acetic acid 50,51 , thus showing that the response is not due to protons but rather is due to the acetate moiety. The intracellular acetate sensor remains to be identified.
We found that ADH activity attributable to ADH2 correlates with ADH2 expression in cells grown with ethanol or acetate as their sole carbon source, although all ADH activity was attenuated in oleic acid grown cells, suggesting further post-transcriptional regulation to occur. It is possible that signaling leading to post translational modification and inhibition of the ADH enzymes may arise from the cleavage and processing of Mga2/Spt23 54 , or as a consequence of changes to membrane fluid dynamics activating sensors such as Mid1 and Mid2 55,56 . Although oleic acid does not affect ADH2 or YRO2 expression, it is probable that other inhibitory relationships exist between other carbon sources, which in most studies are masked by an overall glucose repression. However, under conditions where glucose becomes limiting, such mechanisms of metabolic repression are likely to be of significance.
We then utilized the gene expression of ADH2 and YRO2 to determine whether acetate is sensed extracelullarly, like glucose or osmotic stress, or whether acetate needs to enter the cell in order to elicit a response. Deletion of all three acetate transporters was required to restore ADH2 expression in acetate, and Haa1-GFP did not localize to the nucleus, nor was YRO2 expressed, in strains lacking these transporters when exposed to acetate, although slowing the efflux of acetate to acetyl-coA by deletion of ACS1 did partially restore YRO2 expression.
Since acetate is sensed intracellularly, we reasoned that metabolically produced acetate would also elicit an acetate response, albeit to a lesser degree than upon growth with acetate as the sole carbon source. Indeed, the expression of ADH2 or YRO2 was unaffected in a strain deleted for the acetate transporters when cells were grown on either glucose or ethanol as their sole carbon source, further confirming that the effects in acetate media of these deletions is due to a lack of acetate uptake. We further found that mutations in the metabolic pathway that increase acetate production or lower its utilization increase the acetate response, whereas mutations that lower conversion of acetylaldehyde to acetate lower the acetate response, the major enzyme responsible being ALD6.
Strikingly, all the metabolites involved in this pathway are toxic 21,22,52,57,58 , and yet acetate is produced faster than it is consumed (thus leading to the acetate response in glucose and ethanol grown cells). However, the consequences of upregulating acetyl-coA production may not be benign, as cytoplasmic acetyl-coA is a regulator of autophagy 59 and directly correlates to histone acetylation levels 60 . Therefore the yeast response seems to be not to promote acetate metabolism, but rather to deal with the resultant stress 27,49,61,62 , and as shown here, to reduce expression of ADH2. Together, our results uncover a mechanism by which acetate inhibits its own production, resulting in homeostasis of acetate levels (Fig. 4).

Materials and Methods
Strains used are listed in Table S1; plasmids used are listed in Table S2. All strains are related to BY4741 63 except for W303-1b and V5. Standard yeast molecular biology techniques were used for yeast manipulations. To overexpress ALD6, ALD6-GFP was inserted into ycpADH1 64 digested with SalI/SpeI together with the Cyc1 terminator from pUG34. To make the ACS2-LacZ reporter plasmid, the ZWF1 promoter was excised from a plasmid containing prZWF1::LacZ in Ycp50 65 by BamHI/HindIII digestion, and replaced with 1000 bp of the ACS2 promoter by gap repair.
Media was prepared with 8 g/litre YNB, with 0.0286 g/liter adeneine, tryptophan, histidine, arginine, methionine, 0.0714 g/liter phenylalanine, 0.0857 g/liter tyrosine, lysine, 0.114 g/liter isoleucine, 0.143 g/liter glutamate, aspartate, 0.214 g/liter valine, 0.286 g/liter threonine, 0.571 g/liter serine to make synthetic -LU media. Standard carbon source concentrations were 4% for glucose, 2% for ethanol, 3% for glycerol, 2% for acetate (as sodium acetate at pH6), and 0.14% oleate (in 1% tween-80) 66 . The pH of the resultant media were 4.7 for glucose, 4.7 for ethanol, 4.5 for oleate and 6 for acetate. (In contrast, glucose containing media with 60 mM acetic acid 51 has a pH of 3.9.) The pH of the media after 24 h of yeast growth was 3.5 for glucose, 4.7 for ethanol, 4.5 for oleate and 6 for acetate. Ethanol containing medium was buffered with potassium phosphate at pH6 where indicated in Fig. 1a.
For ADH2 induction assays, cells were grown in 4% glucose overnight to ensure complete repression of ADH2 expression, diluted in the morning and grown for an additional 3 hours (t = 0), washed 3x with water, and resuspended in media containing indicated carbon sources for a further 3 hours (t = 3). The data presented is the t = 3 − t = 0/3 and normalized to the WT ethanol sample from that experiment. Typical ADH2 expression was 1500 Miller Units per hour. For YRO2 and other gene expression assays, cells were taken from glucose plates and grown in 5 ml of indicated media for 24 hours without dilution, to prevent loss of secreted metabolites from the medium. Cells visibly grew during this period.
β-galactosidase assays. β-galactosidase assays were performed using log phase cells. Cell concentration was determined by reading 80 μl of cells at 595 nm. 20 μl of cells were added to the β-galactosidase reaction mix (40 μl YPER (Pierce 78990), 80 μl Z-buffer (120 mM Na 2 HPO 4 , 80 mM NaH 2 PO 4 , 20 mM KCl, 2 mM MgSO 4 ), 24 μl ONPG (4 mg/ml), 0.4 μl β-mercaptoethanol) and incubated at 30 °C for 10 minutes for ADH2, 25 minutes for YRO2 and for 15 minutes for other genes. Reactions were stopped by addition of 56 μl 1 M Na 2 CO 3 . The eppendorf tubes were centrifuged for 1 minute at full-speed to pellet the cell debris, and 200 μl supernatant was removed and absorbance read at 415 nm using a microplate reader. Miller Units were calculated by the equation Miller Units = (1000*A 415 )/(time*0.02*A 595 − 0.055, where the A 415 and A 595 has been corrected for blanking and www.nature.com/scientificreports www.nature.com/scientificreports/ path length (final path length = 1 cm). Three biological replicates were measured. Error bars are ± 1 standard deviation.
Microscopy. 5 μl of log phase cells were imaged using an EVOS microscope (60x objective) with the GFP filter for GFP and the Texas Red filter for Cherry. The dimensions of each panel corresponds to 20 μm × 20 μm. Cells were not concentrated before imaging, to prevent perturbation to their environment 67 . Images were processed using the brightness/contrast function of Image J, to give a black background. For statistics, over 200 cells were counted. Experiments were repeated at least three times on different days. All figures shown in the manuscript are of identical magnification, a size bar is provided in Fig. 3a. ADH assays. Cells were grown overnight in their respective media. 0.8OD 600 was harvested and vortexed with glass beads for 20 minutes at 4 °C with 100 μl PBS + 0.1% Triton X-100 with Protease (Roche) and Phosphatase (Pierce) inhibitors. Supernatant was centrifuged at 4 °C for 10 minutes at 13000 g. An ADH assay kit (Sigma MAK053) was used to determine ADH activity -all volumes were halved from the values suggested in the datasheet, and the amount of developer reduced by half again. Reactions were rapid and completed within 5 minutes. An adh2Δ strain was included as a control. ADH activity is shown as milliUnits per OD 600 of yeast.