Experimental designs for 2G hypergravity and habitation data in mice. (A) The images showed a newly developed gondola-type centrifuge with a 1.0 m arm. (B) The video imaging on before continuous centrifugation of cages, day 1, day 3 and day 14 under the centrifugation. Mice walked in the cage freely before centrifugation on day 0. Mice moved slowly and lay face down on day 1. On day 3, mice gradually adjusted to 2G circumstance and walked in the cage. Mice finally walked freely in the cage on day 14. (C) The changes of body weight in 2G hypergravity experiment. Data are expressed as the mean ± SEM of 7–8 mice. A significant difference between the two groups was indicated; *P < 0.05, **P < 0.01 and ***P < 0.001 vs 1G.