Schematic detailing the interrogation of tumour on-chip to inform patient treatment. Tissue from surgical resection is sliced, generating precision cut tumour slices (PCTS), which are loaded into tumour-on-a-chip devices (A) and treated with radiation based regimens. (B) After treatment PCTS are analysed using specific markers and compared to controls. (C) Results fed back to clinicians could provide information to develop personalised regimens. Solid lines detail methodology employed within the study, dotted lines indicate workflow for development.