Neo open reading frame peptides (TCGA cohort) converge on common peptide sequences. Graphical representation in an isoform of TP53, where amino acids are colored distinctly. (a) somatic single nucleotide variants, (b). positions of frame shift mutations on the −1 and the +1 frame. (c) amino acid sequence of TP53. (d) Peptide (10aa) library (n = 1,000) selection. Peptides belonging to −1 or +1 frame are separated vertically (e,f) pNOPs for the different frames followed by all encountered frame shift mutations (rows), translated to a stop codon (lines) colored by amino acid.