Parameter estimation for platelet aggregometry data obtained by Biola. Estimation of five model parameters and initial platelet concentration (Table 1) was conducted automatically either as in Fig. 2 (“single”), or with both light transmission curve and mean aggregate size as input experimental data (“dual”). For each set of experimental data, parameters of the models were estimated independently. (A) washed platelets, stimulation with 2.5, 5, 10 or 20 µM of ADP, experimental curves were taken from the same blood sample of the same donor, typical experiment out of n = 3 for this donor and out of n = 4 different donors; (B) calculated time-course of the mean size of aggregate (in platelets) for the same models (“single”) as on panel (A); (C) experimental and calculated time-course of the mean size of aggregate for the same conditions (models “dual”) as on panel (A); (D–F) Correlation of the probability of (D) new aggregate formation, (E) single platelet detachment from an aggregate, (F) formation of one cluster from two existing, for dataset from panel (A), models “dual”. (G) Table of Pearson correlation coefficients for this set of data (models “single”).