Figure 3 | Scientific Reports

Figure 3

From: Quantitative dynamics of reversible platelet aggregation: mathematical modelling and experiments

Figure 3

Flow cytometry estimation of aggregate sizes during aggregometry test. Aggregation of washed loaded with Fura Red human platelets in Tyrode’s calcium buffer (3·105/µl) in the presence of 200 µg/ml human fibrinogen was stimulated with 2.5 µM ADP. Samples of the aggregating mixture were taken at points indicated as (A–D) on the aggregation curve (A) and immediately analyzed by flow cytometry. Relative platelet size was measured as ratio of signal in FL-3 (Fura-Red) and mean FL-3 fluorescence of single platelets. One typical aggregation curves out of n = 3 for this donor, similar results were obtained for n = 2 another donors.

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