Cell viability – Cell titre blue and picogreen DNA quantitation. Cell adherence was assessed by CellTiter-Blue® Cell viability assay (A) and further confirmed by Quant-IT™ Picogreen® dsDNA assay (B). Minimum n = 5. Data shown as mean ± 95% confidence interval with statistics performed using One-way ANOVA with Tukey post hoc testing. *p < 0.05 **p < 0.01, ***p < 0.001. Each condition maintains cell survival and the number of cells increases significantly between each time point (A). A significant increase in fluorescence is observed between the polymer only and hLECM scaffolds at day 5 indicating that the presence of human liver ECM has a positive influence on the early expansion and/or survival of hepatocytes. A consistent pattern is observed in the DNA concentration (B) on the scaffolds.