Figure 7 | Scientific Reports

Figure 7

From: Cardiac inflammatory CD11b/c cells exert a protective role in hypertrophied cardiomyocyte by promoting TNFR2- and Orai3- dependent signaling

Figure 7

TNFR2 and Orai signaling pathways promote resistance to oxidative stress of cardiomyocytes after inflammatory stimulation with TNFα or Cmed from in vitro LPS-activated cardiac CD11b/c cells. (A) CD11b/c cells were isolated from normal hearts and further subjected to in vitro activation by LPS incubation following or not semapimod pretreatment. Conditioned media (recovered after 18 hrs in culture) or TNFα were applied on in vitro iso hypertrophied rat cardiomyocytes before H2O2 treatment and analyses of cell resistance. (B) TNFα or Cmed in vitro increase resistance of hypertrophied cardiomyocytes to H2O2 stress in a manner sensitive to semapimod, TNFR2-Ab or YM58483. Mean ± SEM of 3–5 experiments, cardiomyocytes from 3–5 rats, 2–4 Cmed. Kruskal-Wallis followed by Dunn’s post-hoc test vs. the Iso-treated control (white column), **p < 0.01.