Figure 3 | Scientific Reports

Figure 3

From: Cardiac inflammatory CD11b/c cells exert a protective role in hypertrophied cardiomyocyte by promoting TNFR2- and Orai3- dependent signaling

Figure 3

Activation by TNFα relies on binding to TNFR2, stimulation of cPLA2 and potential production of AA metabolites via the lipoxygenase pathway. (AC) Iso-treated hypertrophied cardiomyocytes were loaded with Fura2 before measurement of voltage- and store-independent Ca2+ influx. (A) Rates of Ca2+ entry in response to TNFα after a 1 hour preincubation with control IGg2A, neutralizing TNFR1-Ab or TNFR2-Ab. (B) TNFα effect is sensitive to the cPLA2 inhibitor, MAFP, but mimicked by cPLA2 activating peptide PLAP. (C) Activation of Ca2+ entry by TNFα is blunted by preincubation with NDGA (lipoxygenase inhibitor), but unaffected by indomethacin (cyclooxygenase inhibitor) or montelukast (leukotriene receptor antagonist) pretreatments. Number of cells analyzed and number of cell isolations (rats) as indicated. Mean ± SEM of cells, Wilcoxon matched-paired tests to examine if the mean of the 2nd rate differs from the 1st one, arbitrarily set as 1, *p < 0.05, **p < 0.01, ***p < 0.001.