Figure 1 | Scientific Reports

Figure 1

From: Cardiac inflammatory CD11b/c cells exert a protective role in hypertrophied cardiomyocyte by promoting TNFR2- and Orai3- dependent signaling

Figure 1

TNFα activates a store- and voltage-independent Ca2+ influx in hypertrophied but not in control cardiomyocytes. Representative recordings of Fura2 fluorescence ratio (F340/F380) in iso-treated hypertrophied (A,B) or control (C,D) cardiomyocytes subjected to 2 successive measurements of the rate of voltage- and store-independent Ca2+ entry and quantification of rates of Ca2+ entry. Two successive applications of the same 1 mM Ca2+ basal perfusion medium gave rise to similar rates of Ca2+ entry, both in hypertrophied (A) and normal cells (C). Addition of TNFα to the second 1 mM Ca2+ perfusion medium enhanced the rate of Ca2+ entry (2nd slope as compared to 1st slope) in hypertrophied cardiomyocytes (B) but not in normal cardiomyocytes (D). Number of cells analyzed and number of cell isolations (rats) as indicated. Mean ± SEM of cells, Wilcoxon matched-paired tests to examine if the mean of the 2nd rate differs from the 1st one, arbitrarily set as 1, ****p < 0.0001.