Figure 7 | Scientific Reports

Figure 7

From: The secreted Ly6/uPAR-related protein-1 suppresses neutrophil binding, chemotaxis, and transmigration through human umbilical vein endothelial cells

Figure 7

SLURP1 suppresses fMLP-induced AKT phosphorylation. (A) Lysate from dHL-60 cells preincubated with control protein (CP, mock purified control protein from the parental strain lacking SLURP1 expression vector) or SLURP1 and exposed to 1 μM fMLP (formyl Met-Leu-Phe tripeptide chemoattractant) for 2 to 20 minutes was probed with anti-phospho-AKT (S-473) & anti-total AKT antibodies. Full length, uncropped blots can be seen in Supplemental Fig. 3. (B) The ratio of phospho-AKT: total-AKT quantified by densitometry is shown. The data presented is an average of three independent experiments. An unpaired t test was used to compare phospho-AKT: total-AKT ratios in CP- and SLURP1-treated dHL-60 cells at each time point.