Figure 2 | Scientific Reports

Figure 2

From: The secreted Ly6/uPAR-related protein-1 suppresses neutrophil binding, chemotaxis, and transmigration through human umbilical vein endothelial cells

Figure 2

SLURP1 suppresses the expression of E-Selectin on HUVEC surface. (A) Comparison of E-Selectin transcript levels in TNF-α-activated HUVEC treated with control protein (CP; mock purified control protein from the parental strain without SLURP1 expression vector) or SLURP1 by QPCR. The data presented is an average of two experiments, each with three replicates. (BD) Flow cytometry showing percentage of E-Selectin-positive cells and their median fluorescence intensity. In each experiment, 30,000 cells were analyzed. The data presented is representative of three independent experiments with at least two replicates in each. An unpaired t test was used to compare the values obtained with CP + TNF-α and SLURP1 + TNF-α treated HUVEC.

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