Time kinetics for EBOV/Mak inactivation efficacy results for the 1:20 dilution of DAL lots 15083E, 16004E, and 16005E at ambient temperature. The values represent the mean ± standard deviation (n = three replicates, one for each DAL lot) of the log10 titer of the positive control (0 minutes contact time) and the post-neutralization samples (0.5, 1, 5, and 10 minutes contact time). Individual viral titers were calculated based on three replicate wells per dilution. The limit of detection (LOD) of the assay was 1.8 log10 TCID50/mL (shown in the plot as a blue line extending from y = 1.8 log10 TCID50/mL). This was due to the cytotoxicity of the DAL dilution to the Vero E6 cells.