Figure 5 | Scientific Reports

Figure 5

From: Targeting mechanotransduction mechanisms and tissue weakening signals in the human amniotic membrane

Figure 5

The effect of cyclic tensile strain and pharmacological agents on Cx43 and AKT protein expression in human amniotic membranes. Term human amniotic membranes from the cervical (CAM) or placental regions (PAM) were subjected to cyclic tensile strain (2% CTS, 1 Hz) for 24 hr, in the presence and absence of 0 or 25 µM AKTi, 50 µM Cx43 antisense (Cx43as) and/or 50 µM Cx43 sense oligodeoxynucleotides (Cx43s). Cx43 and AKT protein levels were examined in unstrained and strained CAM or PAM specimens by western blotting (A,B) and normalisation of Cx43 (C) or AKT (D) to GAPDH by densitometry analysis. Error bars represent the mean and SEM values for 4 to 9 replicates from three separate donors, where *p < 0.05; **p < 0.01 and ***p < 0.001.

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