The effect of cyclic tensile strain and pharmacological agents on Cx43 and AKT-1 gene expression. Term human amniotic membranes isolated from the cervix (CAM) or placenta (PAM) regions were subjected to cyclic tensile strain (2% CTS, 1 Hz) for 4 hr and 24 hr, in the presence and absence of 0 or 25 µM AKTi or 50 µM Cx43 antisense (Cx43as). Gene expression of Cx43 and AKT in CAM (A,C) and PAM specimens (B,D) were presented as ratio values and normalised to unstrained control AM cultured in the absence of the chemical agent. In all cases, error bars represent the mean and SEM values of 10 replicates from three separate donors, where (*, ** or ***) indicates significant comparisons for unstrained and strained CAM or PAM conditions. All other comparisons (not indicated) were not significantly different.