The effect of cyclic tensile strain and Cx43/AKT inhibition on inflammatory mediators. Term human amniotic membranes isolated from the cervix (CAM) or placenta (PAM) regions were subjected to cyclic tensile strain (2% CTS, 1 Hz) for 24 hours, in the presence and absence of 0 or 25 µM AKTi or 50 µM Cx43 antisense (Cx43as). Absolute values for PGE2 release (A) and MMP activity (B) are presented in unstrained and strained AM specimens. The corresponding normalised strained values were expressed as a percentage change of the unstrained controls, with SEM values shown in brackets. Error bars represent the mean and SEM values of 16 replicates from four separate donors, where (*, ** or ***) indicates significant comparisons for unstrained and strained CAM or PAM conditions. All other comparisons (not indicated) were not significantly different.