Figure 3 | Scientific Reports

Figure 3

From: Intergeneric hybrids inform reproductive isolating barriers in the Antarctic icefish radiation

Figure 3

Development of the embryos from the C. aceratus by C. rastrospinosus intergeneric cross. (A) 1 dpf, 2-cell stage. (B) 2.5 dpf, 8-cell stage. (C) 15 dpf, blastula stage. (D) 38 dpf, early somite stage. (E) 48 dpf, brain regionalization stage. (F) 66 dpf, onset of retinal pigmentation. (G) 70 dpf, onset of vasculature formation. (H) 90 dpf, onset of cranio-facial skeletal formation. (I) 118 dpf, branchial arches evident. (J) 139 dpf, first fry. (K,L) 146 dpf, second fry stage. Scale bars represent 1 mm. Embryos in A–C were fixed with Stockard’s solution before imaging. Embryos in D–L were pictured live. Abbreviations: b, brain; e, eye; h, heart; jb, jaw bones; l, lens; ot, otoliths; pfb, pectoral fin bud; t, teeth; y, yolk sac. The white arrow in I and J points at the curvature of the developing snout.

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