Figure 4 | Scientific Reports

Figure 4

From: microRNA-155 inhibition restores Fibroblast Growth Factor 7 expression in diabetic skin and decreases wound inflammation

Figure 4

miR-155 inhibitor significantly increases FGF7 expression and improves keratinocyte migration in vitro and in vivo. (A) FGF7 mRNA levels in skin wound samples from control and diabetic mice (n = 6/group), collected at baseline (Day 0) and at days 3 and 10 post wounding, quantified by RT-qPCR and normalized to the mean of TFIIB and U6 levels and control baseline. (B) Luciferase activity of HaCAT cells transfected with a luc-reporter vector carrying the FGF7 3′ untranslated region (UTR) or with a mutated vector where one (of two) miR-155 binding sites is mutated and 12, 25 or 35 pmol/48 well miR-155 inhibitor oligo. (C) HaCAT cells were transfected with miR-155 inhibitor or scrambled miR (neg control). Scratches were made 48 h post-transfection and measured again 24 h later. Seed 155i and Seed Neg: LNA-oligonucleotides binding to seed region of miR-155 or a negative control. Data were analyzed using two-way ANOVA with Dunnet’s post hoc correction (A) and Student’s t-test (B,C); **p < 0.01; ***p < 0.001.