Figure 2 | Scientific Reports

Figure 2

From: Collagen XXV promotes myoblast fusion during myogenic differentiation and muscle formation

Figure 2

Col25a1 expression during myogenic differentiation. (A) The expression of Col25a1 and Myh7 was analyzed by RT-qPCR from E12.5, E14.5 WT limb buds and E18.5 WT hindlimb muscles. Data are means ± s.e.m of three independent experiments. Three to six embryos were analyzed in each experiment. ** indicates P < 0.01; ***P < 0.001 vs E12.5 limbs. (B) Juvenile satellite cells were isolated from WT 10 day old mice and expanded. The expression profile of Col25a1, myogenin and Myh3 was analyzed by RT-qPCR in cells cultured in proliferation medium (PM), or 2 (DM2), 3 (DM3) or 4 (DM4) days in differentiation medium. Data are means ± s.e.m of three different experiments. * indicates P < 0.05; **P < 0.01; ***P < 0.001 vs PM.