Figure 8 | Scientific Reports

Figure 8

From: Role of vimentin in modulating immune cell apoptosis and inflammatory responses in sepsis

Figure 8

Release of cytokines in the supernatants. Jurkat cells were transfected with control siRNA, vimentin-specific siRNA or a plasmid expressing vimentin as described in the Materials and Methods. Cells were then cultured in serum-free DMEM in the presence or absence of LPS (10 µg/mL) for 24 h. Supernatants were harvested and used for quantification of cytokines by ELISA as described in the Materials and Methods. Panel A: TNF-α. Panel B: IL-2. Panel C: IL-10. Panel D: IFN-γ. Vertical axes: cytokine level expressed as pg/day/105 cells; horizontal axes: cells transfected with control siRNA (Con-siRNA), vimentin-specific siRNA (Vim-siRNA) or the plasmid expressing vimentin (Vim-vector). Data are shown from one representative assessment; experiments were each repeated at least 3 times with similar results. Open bar: cells cultured in the serum-free DMEM (SF-DMEM); closed bar: cells treated with 10 µg/mL LPS. *p < 0.05 compared to cells cultured in SF-DMEM; #p < 0.05 compared to cells transfected with Con-siRNA and cultured in SF-DMEM.