Figure 6 | Scientific Reports

Figure 6

From: Role of vimentin in modulating immune cell apoptosis and inflammatory responses in sepsis

Figure 6

Role of vimentin in modulating Jurkat cell apoptosis in response to LPS. Jurkat cells were transfected with control siRNA, vimentin-specific siRNA or a plasmid expressing vimentin as described in the Materials and Methods. Cells were then cultured for 6, 18 and 24 h in the presence or absence of LPS (10 µg/mL). Panel A: Representative images of TUNEL staining in the presence of LPS. Panel B: Quantitative comparison of TUNEL positivity in cells with or without LPS exposure. *p < 0.05 compared to control cells without LPS; #p < 0.05 compared to control cells with LPS. Data shown are from a single representative assessment; the experiments were repeated at least 3 times with similar results.