Figure 5 | Scientific Reports

Figure 5

From: Role of vimentin in modulating immune cell apoptosis and inflammatory responses in sepsis

Figure 5

Vimentin expression in Jurkat cells and its effects on apoptosis. Jurkat cells were transfected with control siRNA, vimentin-specific siRNA or a plasmid expressing vimentin as described in the Materials and Methods. Cells were cultured in serum-free DMEM as indicated, followed by TUNEL assay analysis as described in the Materials and Methods. Panel A: Representative immunoblot indicating suppression of vimentin by siRNA (Vim-siRNA) or overexpression of vimentin (Vim-DNA). Panel B: Representative images of TUNEL assays in the absence of LPS. Green: TUNEL-positive staining; Red: cell nuclei.