Analysis of the regulatory network of Lrrc75a-as1. (A,B) Expression change of (A) miR-29a-3p and (B) miR-24-3p during vascular calcification, and their sequences predicted to bind to that of Lrrc75a-as1 are shown. The expression change of miRNAs between untreated and Pi-treated samples for three or six days was calculated. The binding position was predicted from miRNA_Targets server38. (C) Analysis of transcription factors in the promoter of human Lrrc75a-as1. The position of ChIP-seq signals for each transcription factor and H3K27Ac was obtained using the UCSC Genome Browser (http://genome.ucsc.edu)58. The histone marker H3K27Ac is usually associated with active enhancers59.