Figure 3 | Scientific Reports

Figure 3

From: Long noncoding RNAs in vascular smooth muscle cells regulate vascular calcification

Figure 3

Characterization of candidate lncRNAs. (A) Genomic information of selected lncRNAs. By performing 5′ and 3′ RACE experiments and subsequent sequencing, the transcription start and end positions, and splice junctions were identified. The nucleotide position is based on rn6 (July 2014) genome assembly. Yellow boxes represent the exons of lncRNAs. Blue boxes represent the locus of nearby protein-coding genes. (B) The subcellular distribution of lncRNAs. LncRNA levels were measured in the nuclear and cytoplasmic fractions of A10 cells. Results showed that Linc00116, Lrrc75a-as1, and Snhg16 were mainly localized in the cytoplasm. Snhg1 was localized in the nucleus. Malat1 and pre-Gapdh were used as nuclear controls, while mature Gapdh and Actb were used as cytoplasmic markers. The error bars indicate standard error of triplicate measurements (n = 3). (C) Protein-coding potential of lncRNAs. The protein-coding potential of candidate lncRNAs was evaluated by CPC36 and CPAT37.

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