Identification of lncRNAs involved in vascular calcification. (A) Experiment scheme. After splitting the primary-cultured rat VSMCs into four dishes, they were treated with 2 mM Pi for different time periods. Time point of Pi treatment was adjusted such that all cells had the same growth period. (B) Schematic representation of lncRNA analysis. RNA sequencing and its following analyses steps are illustrated. Refer to the Materials and methods section for details. (C) Three criteria to select the candidate lncRNAs: differential expression of lncRNAs among the samples, conservation among species, and existence of near gene in genomic context with known function related with vascular calcification. Refer to the main text for details.