Figure 3 | Scientific Reports

Figure 3

From: High Skp2 expression is associated with a mesenchymal phenotype and increased tumorigenic potential of prostate cancer cells

Figure 3

DU 145 cells with a mesenchymal phenotype are considered cancer stem-like cells. (A) Analysis of CD44 and CD24 markers in mesenchymal and epithelial DU 145 cells using flow cytometry. Dot plots show representative results from three independent experiments. (B) Quantitation of results shown in (A). Bars show the mean ± SD of the percentage of CD44+CD24 cancer stem-like cells (n = 3). (C) Spheroid formation and growth in anchorage-independent conditions in DU 145 mesenchymal and epithelial cells. (D) Quantitation of results shown in (C). Data are shown as the mean ± SD of the number of spheroids per well. Results are from three independent repetitions. (E) ALDH1 activity in DU 145 mesenchymal and epithelial sublines. Data are presented as the mean ± SD of the percentage of cells with active ALDH1. Results are from three independent repetitions.