Figure 5 | Scientific Reports

Figure 5

From: The pro-angiogenic role of hypoxia inducible factor stabilizer FG-4592 and its application in an in vivo tissue engineering chamber model

Figure 5

The influence of FG-4592 on expression of PHD-HIF-VEGF axis and pro-inflammatory cytokines. HUVECs or RAW264.7 were pretreated with 0 uM or 20 uM FG-4592 before samples were collected. (A) Western blot analysis of HIF-1α and VEGF protein expression in HUVECs after FG-4592 treatment. The relative expression of HIF-1α and VEGF was normalized to β-Actin. (B) Besides, Q-rtPCR analysis of HIF-1α and VEGF was performed. The relative mRNA expression was normalized to β-Actin and the expression of negative control group served as a reference, which was set as 1. (C) Similarly, Q-rtPCR analysis of TNFα and IL1β was performed to detect their expression in RAW264.7. *p < 0.05, **p < 0.01.