Figure 2 | Scientific Reports

Figure 2

From: Scale invariance of BMP signaling gradients in zebrafish

Figure 2

P-Smad5 gradient profiles of WT and Cut scale. (a) The animal and lateral views of P-Smad5 stained 6 hpf embryo point clouds after image processing with P-Smad5 labeling intensity in color. The animal and lateral view of a P-Smad5 stained WT embryo (Left) displays a similar P-Smad5 gradient as the Cut embryo (Middle). The animal and lateral view of a P-Smad5 stained Cut embryo (Right). (b) The boxplot of relative egg sizes of P-Smad5 stained embryos. All Cut are all of the Cut embryos imaged. Cut Compared are the Cut embryos whose difference of P-Smad5 relative intensity between ventral and dorsal half is no less than 0.2 after normalization. From left to right, they are all WT (N = 24), All Cut (N = 50) and Cut Compared (or just ‘Cut’ for simplication) (N = 20). (cf) P-Smad5 gradient profiles of WT (black) and Cut (red) embryos after filtration. How x/Lmax vs x/L plots are normalized is described in Materials and Methods, Computational Image Analysis section. (c) Population mean P-Smad5 gradients of WT and Cut after filtering on x/Lmax plot with variation shown by error bar. (d) Population mean P-Smad5 gradients of WT and Cut on x/L plot with variation shown by error bar. (e) P-Smad5 signal of embryos grouped by 10° interval in dots on x/Lmax plot, WT in black, Cut Compared in red. (f) P-Smad5 signal of each embryos averaged by 10° interval in dots on x/L plot, WT in black, Cut Compared in red. Each dot corresponds to an average of P-Smad5 signals of all cells within every 10° along the margin within one embryo. (gi) Fitted curves and point-wise SEs for population mean morphogen gradients of Cut and WT on x/L plot and x/Lmax plot. (g) SE of Cut and WT population mean morphogen gradients on x/Lmax plot (blue) vs on x/L plot (magenta). (h) Curve fitting of population P-Smad5 gradients on x/Lmax plot. Average P-Smad5 signals of every 10° along the margin of each WT embryo are fitted with a smooth curve using the Hill equation (equation 1) and plotted as a black line on x/Lmax plot. (i) Curve fitting of population P-Smad5 gradients on x/L plot. Average P-Smad5 signals of every 10° along the margin of each WT embryo are fitted with a smooth curve using the Hill equation (equation 1) and plotted as a black line on x/L plot. (jl): Ratio of curve-fit parameters vs L along the cut and WT embryo margins. (j) Scatter plot of the curve-fit parameter a vsL fitting with a line in blue, used to calculate the scaling power of parameter a. (k) Scatter plot of the curve-fit parameter b vs L fitting with a line in blue, used to calculate the scaling power of parameter b. (l) Scatter plot of the curve-fit parameter c vs L fitting with a line in blue, used to calculate the scaling power of parameter c.

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