Phenotype distributions of cut embryos. (a) The bar plot shows the frequencies of different phenotypes of Cut larvae at 1 dpf. Normal: DV proportions same as wild-type. C1~C5: dorsalized phenotypes from weakest to strongest, with the proportions of DV axis tissues different from WT or some tissues along DV axis missing. The definitions of these 5 classes of phenotypes are used as in Mullins et al. C1: no tail fin. C2: no tail vein. C3: no yolk extension. C4: no tail. C5: the yolk spills out of the embryo and the embryo lyses1. The number of embryos that look ventralized is also combined to Class 5 category. (b) Lateral view of live WT (top left two) and Cut embryos at 6 hpf and 24 hpf under bright-field microscope. Embryos displaying normal, C1, C2, C3, C4, C5(the embryo lyses) and ventralized (no head) phenotypes are imaged at 6 hpf and 24 hpf with phenotype labels on the 24 hpf image panel.