Temperature induces changes in Drosophila fat stores. (A,B) Absolute fat content (µg glycerides per fly) in Oregon R (A), and Canton S flies (B). (C,D) Relative changes in the absolute fat content in Oregon R (C), and Canton S flies (D). The values were calculated as a ratio between the final value and the initial value of fat content for a given biological replicate. Data were compared to the values at 25 °C (red) by Welch’s t-test with the Benjamini-Hochberg correction for multiple testing. Individual data points represent biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001. For global statistical analyses see Supplementary Table S2.