Table 3 Recovery (%) of BoNT from complex matrices at low, medium or high concentration range in the Luminex duplex-assays.

From: Functional detection of botulinum neurotoxin serotypes A to F by monoclonal neoepitope-specific antibodies and suspension array technology

Matrix Toxin Lowa,b Mediumb Highb
Serum BoNT/A 58 ± 25.8 85 ± 13.4 99 ± 1.8
BoNT/B 45 ± 3.1 58 ± 10.7 92 ± 1.7
BoNT/C 156 ± 30 100 ± 7.9 98 ± 3.7
BoNT/D 89 ± 11.9 89 ± 7.1 98 ± 1.9
BoNT/E 40 ± 5.0 34 ± 7.2 96 ± 1.5
BoNT/F 83 ± 4.2 81 ± 7.0 99 ± 2.7
Fish BoNT/A 76 ± 13.5 93 ± 29 100 ± 1.8
BoNT/B n.d.c 11 ± 8.8 11 ± 1.7
Sausage BoNT/A 62 ± 21.4 51 ± 6.9 96 ± 5
BoNT/B 78 ± 25.6 48 ± 21.9 74 ± 22.9
Beans BoNT/A 113 ± 32.2 95 ± 18.8 97 ± 3.4
BoNT/B 72 ± 31 43 ± 14.8 85 ± 12.5
  1. aSpiked low toxin concentrations in serum for BoNT/A to F: 7 (A), 5 (B), 2000 (C), 2 (D), 70 (E), 2 (F) pg/mL. Medium toxin concentrations were near the EC50 value, low toxin concentration: EC50/10 and high toxin concentration: EC50 × 10. For detection from food matrices, the low concentration was adjusted to 15 pg/mL for BoNT/A and 5 pg/mL for BoNT/B. bToxin recovery from spiked serum samples compared to spiked BSA/PBS as reference (100%) was calculated. Results from two independent experiments, each performed in technical duplicates (n = 4; mean ± SD). cn.d. = not detectable.