Figure 2 | Scientific Reports

Figure 2

From: Functional detection of botulinum neurotoxin serotypes A to F by monoclonal neoepitope-specific antibodies and suspension array technology

Figure 2

Selected neoepitope specific monoclonal antibodies exclusively recognise the specific cleavage site of the corresponding BoNT. (a) Western blot results to compare detection of cleaved vs. uncleaved SNAP-25 or VAMP-2 by the Neo-mAbs. SNAP-25 or VAMP-2 was incubated with (+) or without (−) BoNT overnight at 37 °C in cleavage buffer, samples were loaded on two identical polyacrylamide gels of which one gel was used for Coomassie staining, and the second gel was applied for Western blotting using Neo-mAbs. (b) Endopep-ELISA demonstrating specific recognition of cleavage sites. VAMP-2 or SNAP-25 were coated on microtitre plates, cleaved with 10 ng/mL BoNT as indicated and cleavage products were detected with Neo-mAbs indicated above each panel. Results from two independent experiments, each performed in technical duplicates are shown (n = 4; mean ± SD).

Back to article page