Figure 6 | Scientific Reports

Figure 6

From: Neural anti-inflammatory action mediated by two types of acetylcholine receptors in the small intestine

Figure 6

Inhibition of M2AChR prevented the anti-inflammatory action of 5-HT4R stimulation against neutrophil infiltration, but not macrophage infiltration, in POI models. (A and B) Effect of the nonselective mAChRs antagonist atropine on mosapride citrate (MOS)-induced anti-inflammatory action against CD11b+Gr-1 macrophage infiltration (A) and Gr-1+Ly6C+ neutrophil infiltration. Atropine (Atr; 3 mg/kg) was subcutaneously administered 10 min before each application of MOS, and *** indicates values significantly different from control at p < 0.001, and # indicates values significantly different from IM at p < 0.05 (n = 4 each). Each column shows the mean ± SEM. (C) Effects of specific mAChR antagonists on the MOS-induced anti-inflammatory action against Gr-1+Ly6C+ neutrophil infiltration in postoperative ileus. The M1AChR antagonist pirenzepine (Pire; 1 mg.kg), the M2AChR antagonist methoctramine (1 mg/kg), and the m3AChR antagonist 4-DAMP (1 mg/kg) were subcutaneously administered 10 min before each application of MOS, and *** indicates values significantly different from control at p < 0.001, and # indicates values significantly different from IM at p < 0.05 (n = 4 each). (D) MOS-induced anti-inflammatory action against Gr-1+Ly6C+ neutrophil infiltration in postoperative ileus models of M2AChR KO (M2R KO) mice, where *** indicates values significantly different from control at p < 0.001, and n.s. indicates non-significant values.

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