Figure 4 | Scientific Reports

Figure 4

From: Neural anti-inflammatory action mediated by two types of acetylcholine receptors in the small intestine

Figure 4

The α7nAChR selective agonists PNU and AR-R inhibited macrophage infiltration, but not neutrophil infiltration, in postoperative ileus models of WT mice. Effects of α7nAChR stimulation on leukocyte infiltration by intestinal manipulation (IM) in postoperative ileus models of WT mice. PNU- (PNU) or AR-R- (AR-R) were subcutaneously administered as described in Methods. (A) Immunohistochemical staining of CD68-positive macrophages in postoperative ileus of WT mice. Representative images from 4 independent experiments are shown. Bar indicates 100 μm. (B) Quantified results of infiltrated macrophage cell numbers from A, where * or *** indicates values significantly different from control at p < 0.05 or 0.001 (n = 4 each), respectively. Each column shows the mean ± SEM. (C) Effect of PNU or AR-R on increased MPO activity of inflamed intestinal muscle layer in postoperative ileus of WT mice. Bars show the means ± SEM (n = 4–6). (D) Quantitative results of infiltrated neutrophil cell numbers from histochemistry results. Each column shows the mean ± SEM (n = 4).

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