Figure 3 | Scientific Reports

Figure 3

From: Neural anti-inflammatory action mediated by two types of acetylcholine receptors in the small intestine

Figure 3

Inhibitory action of 5-HT4R stimulation against macrophage infiltration, but not neutrophil infiltration, was attenuated in postoperative ileus models of α7 R KO mice. Effects of 5-HT4R stimulation on leukocyte infiltration by intestinal manipulation (IM) in postoperative ileus models of α7 R KO mice. (A) Immunohistochemical or histochemical staining of CD68-positive macrophages or MPO-stained neutrophils in α7 R KO mice intestines. Representative images from 4 independent experiments are shown. Bar indicates 100 μm. Immunohistochemical conditions were identical for Fig. 1A. (B and C) Quantified results of infiltrated macrophage (B) and neutrophil (C) cell numbers from A, where *** indicates values significantly different from control at p < 0.001 (n = 4 each), and ## indicates values significantly different from IM at p < 0.01, respectively (n = 4 each). Each column shows the mean ± SEM. (D) Typical results of flow cytometry to detect CD11b+Gr-1 macrophage subsets (upper trace) or Gr-1+Ly6C+ neutrophil subsets in POI models of α7 R KO mice. Representative results of flow cytometry from 4 independent experiments are shown. (E and F) Quantified results of % of CD11b+Gr-1 macrophage subsets (E) and Gr-1+Ly6C+ neutrophil subsets (F) of total leukocytes, where *** indicates values significantly different from control at p < 0.001 (n = 4 each), and ## indicates values significantly different from IM at p < 0.01, respectively (n = 4 each). Each column shows the mean ± SEM.

Back to article page