Cytotoxicity and intracellular localization of FITC-labelled NLS18 and NLS23 associated to TEAD/YAP interfering peptides. (a) MDA-MB-231 cells were incubated 4 h with NLS18-TEAD peptide. Toxicity was analysed by flow cytometry. Non-treated cells were used as control. (b) Peripheral blood mononuclear cells (PBMC) were incubated 4 h with NLS18-TEAD or NLS23-TEAD peptides. Toxicity was analyzed as above. Non-treated cells were used as control. (c) MDA-MB231 cells (3 × 104 cells/ml) were incubated in Labtek chamber slides cover slips for 3 h with 15 μΜ of the FITC-labelled NLS18 and NLS23 associated to a cargo, the interfering peptide blocking the interaction between TEAD and YAP proteins and vice versa. Cells were washed 3 times with PBS, fixed with 4% PFA and analyzed by fluorescence microscopy.