Table 2 Sequencing prep master mix.

From: Demonstration of End-to-End Automation of DNA Data Storage

Reagent Volume (μL)
Extended adapter 15
T4 DNA ligase (NEB: M0202) 5
DTT-free 10× T4 buffer* 20
ONT RBF 93
Nuclease-free water 64
Total 197
  1. *DTT-free 1× T4 buffer: 50 mM Tris-HCl, 10 mM MgCl2, 1 mM ATP.