Table 1 DNA synthesis reagent parameters.

From: Demonstration of End-to-End Automation of DNA Data Storage

Step Volume (μL) Time (s)
deblock 600 50
Act + {A, C, T, G} (1:1) 350 120
Act + Phos. reagent (1:1)* 350 900
Oxidizer 750 10
  1. *Only performed as final coupling step to add 5′ phosphate.