Table 1 Ingredient and proximate composition (g/kg) of the experimental diets.

From: Freshwater microalgae (Schizochytrium sp.) as a substitute to fish oil for shrimp feed

Ingredients Experimental diets
1 2 3 4 5 6 7 8
Fishmeal 250 250 250 250 250 250 250 250
Soybean meal 397 397 397 397 397 397 397 397
Wheat meal 180 176 172 168 164 159 159 156
Wheat gluten 80 80 80 80 80 80 80 80
Fish oil 50 25 13 0 0 0 0 0
Linseed oil 0 0 0 4 8 8 0 0
Soybean oil 0 17 21 21 8 0 8 0
Algae meal (66% oil) 0 12 25 37 50 62 62 75
Cholesterol 2 2 2 2 2 2 2 2
Lecithin 5 5 5 5 5 5 5 5
DCP 15 15 15 15 15 15 15 15
MgSO4 6 6 6 6 6 6 6 6
KH2PO4 8 8 8 8 8 8 8 8
Vitamin C 1 1 1 1 1 1 1 1
Vitamin premixa 4 4 4 4 4 4 4 4
Mineral premixb 1 1 1 1 1 1 1 1
Choline chloride 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5
Proximate composition (g/kg dry matter)
Moisture 136 116 123 109 121 122 117 117
Crude protein 407 384 381 377 385 389 392 396
Crude lipid 82 85 85 84 87 90 94 89
Crude ash 108 109 112 109 108 111 108 110
  1. aProvides per kg of diet: retinyl acetate (vitamin A), 3000 IU; cholecalciferol (vitamin D), 2400 IU; all-rac-α-tocopheryl acetate (vitamin E), 60 IU; menadione sodium bisulfite (vitamin K), 1.2 mg; ascorbic acid monophosphate (49% ascorbic acid, vitamin C), 120 mg; cyanocobalamine (vitamin B12), 0.024 mg; d-biotin, 0.168 mg; choline chloride, 1200 mg; folic acid, 1.2 mg; niacin, 12 mg; d-calcium pantothenate, 26 mg; pyridoxine, HCl, 6 mg; riboflavin, 7.2 mg; thiamin. HCl, 1.2 mg.
  2. bProvides per kg of diet: sodium chloride (NaCL, 3%Na, 61%CL), 3077 mg; ferrous sulfate (FeSO4·7H2O, 20% Fe), 65 mg; manganese sulfate (MnSO4, 36% Mn), 89 mg; zinc sulfate (ZnSO4·7H2O, 40% Zn), 150 mg; copper sulfate (CuSO4.5H2O, 25% Cu), 28 mg; potassium iodide (KI, 24% K, 76% I), 11 mg; celite AW521 (acid-washed diatomaceous earth silica, 1000 mg).