Figure 2 | Scientific Reports

Figure 2

From: Gadd45b mediates depressive-like role through DNA demethylation

Figure 2

Behavioral effects of local deletion of Gadd45b in NAc on social avoidance behaviors. (a) Schematic diagram depicting the experimental procedures for CSDS and intra-NAc infusion of HSV-Gadd45b miR. (b) Immunohistochemistry after HSV-Gadd45b miR infusion into NAc. (c) HSV-Gadd45b miR infused into NAc significantly decreases Gadd45b mRNA levels in NAc (t(15) = 2.405, *p < 0.05, HSV-GFP n = 8, HSV-Gadd45b miR n = 9). (d–f) Local Gadd45b KD in the NAc had no effect on social interaction in stress naïve animals [d, Mixed model two-way ANOVA, time effect: F(1,32) = 1.305, p = 0.262; genetic effect: F(1,32) = 0.142, p = 0.709; time × genetic effect: F(1,32) = 0.028, p = 0.868, HSV-GFP (pre- and post-) n = 9, HSV-Gadd45b miR (pre- and post-) n = 9]. In contrast, local Gadd45b KD in this brain region reverses the CSDS-induced social avoidance behavior in susceptible mice [e, time effect: F(1,44) = 3.25, p = 0.078; genetic effect: F(1,44) = 1.669, p = 0.203; time × genetic effect: F(1,44) = 8.398, p < 0.01, HSV-GFP (pre- and post-) n = 11, HSV-Gadd45b miR (pre- and post-) n = 13]. After HSV-GFP infusion into NAc, resilient mice showed reduction of social interaction, whereas intra-NAc infusion of HSV-Gadd45b miR blocks this effect [f, time effect: F(1,40) = 6.534, p < 0.05; genetic effect: F(1,40) = 6.913, p < 0.05; time × genetic effect: F(1,40) = 4.411, p < 0.05, HSV-GFP (pre- and post-) n = 11, HSV-Gadd45b miR (pre- and post-) n = 11]. Mixed model two-way ANOVA with Fisher’s LSD post-hoc tests, *p < 0.05, **p < 0.01, ***p < 0.001. Bar graphs show mean ± SEM.

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