Table 4 Stability of candidate reference genes in liver tissue.

From: Selection of suitable reference genes for gene expression analysis in gills and liver of fish under field pollution conditions

Gene geNorm NormFinder Best Keeper Delta Ct Comprehensive ranking
M value Rank Stability value Rank SD [+/−CP] Rank Average of standard deviations Rank Geometric mean of ranking values Rank
rpl8 0.308 1 0.203 1 0.85 1 2.06 3 1.32 1
rpl13 0.308 1 0.433 4 1.04 4 2.05 1 2 2
hprt 0.354 2 0.306 2 0.98 3 2.06 2 2.45 3
actb 0.45 3 0.368 3 0.95 2 2.13 4 3.13 4
tuba 1.954 4 4.193 5 1.42 5 4.64 5 5 5
gapdh 3.015 5 4.814 6 1.9 6 5.14 6 6 6