Table 3 Stability of candidate reference genes in gill tissue.

From: Selection of suitable reference genes for gene expression analysis in gills and liver of fish under field pollution conditions

Gene geNorm NormFinder Best Keeper Delta Ct Comprehensive ranking
M value Rank Stability value Rank SD [+/−CP] Rank Average of standard deviations Rank Geometric mean of ranking values Rank
rpl8 0.192 1 0.188 1 0.67 2 0.4 1 1.19 1
hprt 0.316 2 0.261 2 0.79 6 0.44 2 2.91 2
rpl13 0.192 1 0.32 4 0.77 5 0.46 4 2.99 3
gapdh 0.398 4 0.287 3 0.75 3 0.46 3 3.41 4
tuba 0.481 5 0.584 6 0.57 1 0.65 6 3.83 5
actb 0.383 3 0.36 5 0.77 4 0.48 5 4.47 6