Lifeact expression alters cofilin activity. (a) Lifeact dose dependent effects on F-actin disassembly after 30 min of Latrunculin A (0.075 µg/ml) treatment. (b) Western blot results for cofilin and p-cofilin expression levels relative to GADPH. (c) Lifeact dose dependent effects on fluorescence intensities of cofilin colocalized to F-actin fibres. For (a) and (c), data is presented as median, error bars indicate Q1-Q3, N > 100 cells; for (b) data is presented as mean, error bars indicate standard deviation, N = 3 repeats.