Lifeact expression alters cell migration and F-actin dynamics. Lifeact dose dependent effects on distance migrated (a), directionality of migration (b) and F-actin inter-frame changes (c). Values for >5 cells were pooled to compute each individual data point. Data is presented as geometric mean, error bars indicate geometric standard deviation. Background colours indicate the regimes where cells display no Lifeact-induced effect (yellow background), a dose-response trend (white background) and a saturation plateau (gray background), as identified from analyses of peak changes in variability in the neighbourhood of each point for each parameter plotted.