Lifeact-driven effects modulate nuclear state. Quantification of Lifeact effects on nuclear volume (a) nuclear Poisson’s Ratio (b). Values for >40 cells were pooled together to compute each individual data point. Data is presented as mean, error bars indicate standard deviation. Background colours indicate the regimes where cells display no Lifeact-induced effect (yellow background), a dose-response trend (white background) and a saturation plateau (gray background), as identified from analyses of peak changes in variability in the neighbourhood of each point for each parameter plotted.