Lifeact-driven effects extend to non-actin-based cytoskeletal networks. Quantification of Lifeact effects on intermediate filaments assembly (a) and microtubule assembly (b). Values for >40 cells were pooled together to compute each individual data point. Data is presented as geometric mean, error bars indicate geometric standard deviation. Background colours indicate the regimes where cells display no Lifeact-induced effect (yellow background), a dose-response trend (white background) and a saturation plateau (gray background), as identified from analyses of peak changes in variability in the neighbourhood of each point for each parameter plotted.