Figure 5 | Scientific Reports

Figure 5

From: Isolation and characterization of myogenic precursor cells from human cremaster muscle

Figure 5

Expression of myogenic, endothelial and mesenchymal markers during in vitro expansion of human cremaster muscle-derived myogenic precursor cells. (AC) Immunofluorescence analysis of myogenic protein expression in d7 (A,B) and d15-cultured myospheres (C). Arrows indicate cells positive for PAX7 (green, A), and MYOGENIN (green, B). Confocal microscope image showing PAX7 positive cells (green, C). (D) Percentage of satellite cells (PAX7 positive cells) as quantified in several spheres from three independent biopsies. The blue line represents the median value of the data. (EJ) Serial confocal microscope images of d15-cultured spheres analysed by immunofluorescence for the detection of endothelial cell marker CD31 (EG) and mesenchymal cell marker CD90 (HJ). Nuclei were counterstained with Hoechst 33258 (blue). Scale bars, 100 μm (A,B) and 50 μm (C,EJ).